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Article Dans Une Revue Biomaterials Année : 2021

Comprehensive characterization of the human pancreatic proteome for bioengineering applications

Amish Asthana
  • Fonction : Auteur
Riccardo Tamburrini
Deborah Chaimov
  • Fonction : Auteur
Carlo Gazia
  • Fonction : Auteur
Stephen Walker
  • Fonction : Auteur
Mark van Dyke
Alice Tomei
  • Fonction : Auteur
John Robertson
Emmanuel Opara
  • Fonction : Auteur
Shay Soker
  • Fonction : Auteur
Giuseppe Orlando
  • Fonction : Auteur

Résumé

Interactions between the pancreatic extracellular matrix (ECM) and islet cells are known to regulate multiple aspects of islet physiology, including survival, proliferation, and glucose-stimulated insulin secretion. Recognizing the essential role of ECM in islet survival and function, various engineering approaches have been developed that aim to utilize ECM-based materials to recreate a native-like microenvironment. However, a major impediment to the success of these approaches has been the lack of a robust and comprehensive characterization of the human pancreatic proteome. Herein, by combining mass spectrometry (MS) and multiplex ELISA, we have provided an improved workflow for the in-depth profiling of the proteome, including minor constituents that are generally underrepresented. Moreover, we have further validated the effectiveness of our detergent-free decellularization protocol in the removal of cellular proteins and retention of the matrisome. It has also been established that the decellularized ECM and its derivatives can provide more tissue-specific cues than traditionally used biological scaffolds and are therefore more physiologically relevant for the development of hydrogels, bioinks and medium additives, in order to create a pancreatic niche. The data generated in this study would contribute significantly to the efforts of comprehensively defining the ECM atlas and also serve as a standard for the human pancreatic proteome to provide further guidance for design and engineering strategies for improved tissue engineering scaffolds.
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Dates et versions

hal-04542805 , version 1 (11-04-2024)

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Citer

Amish Asthana, Riccardo Tamburrini, Deborah Chaimov, Carlo Gazia, Stephen Walker, et al.. Comprehensive characterization of the human pancreatic proteome for bioengineering applications. Biomaterials, 2021, 270, pp.120613. ⟨10.1016/j.biomaterials.2020.120613⟩. ⟨hal-04542805⟩

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