Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Crystals Année : 2020

Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering

Raphaël de Wijn
  • Fonction : Auteur
  • PersonId : 1080590
Kévin Rollet
  • Fonction : Auteur
  • PersonId : 1080591
Sylvain Engilberge
  • Fonction : Auteur
  • PersonId : 1080592
Oliver Hennig
  • Fonction : Auteur
  • PersonId : 1080594
Heike Betat
  • Fonction : Auteur
  • PersonId : 1080595
Mario Mörl
  • Fonction : Auteur
  • PersonId : 1080596
François Riobé
Olivier Maury
Eric Girard
  • Fonction : Auteur
  • PersonId : 1080597
Philippe Bénas
  • Fonction : Auteur
  • PersonId : 1080598
Claude Sauter

Résumé

The reproducible preparation of well-diffracting crystals is a prerequisite for every structural study based on crystallography. An instrument called XtalController has recently been designed that allows the monitoring of crystallization assays using dynamic light scattering and microscopy, and integrates piezo pumps to alter the composition of the mother liquor during the experiment. We have applied this technology to study the crystallization of two enzymes, the CCA-adding enzyme of the psychrophilic bacterium Planococcus halocryophilus, and the lysozyme from hen egg white in the presence of a synthetic chemical nucleant. We were able to (i) detect early nucleation events and (ii) drive the crystallization system (through cycles of dissolution/crystallization) toward growth conditions yielding crystals with excellent diffraction properties. This technology opens a way to the rational production of samples for crystallography, ranging from nanocrystals for electron diffraction, microcrystals for serial or conventional X-ray diffraction, to larger crystals for neutron diffraction.
Fichier principal
Vignette du fichier
crystals-10-00065-v3.pdf (4.53 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02464277 , version 1 (29-10-2020)

Identifiants

Citer

Raphaël de Wijn, Kévin Rollet, Sylvain Engilberge, Alastair G Mcewen, Oliver Hennig, et al.. Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering. Crystals, 2020, 10 (2), pp.65. ⟨10.3390/cryst10020065⟩. ⟨hal-02464277⟩
329 Consultations
138 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More