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The AAA+ ATPase ATAD3A Controls Mitochondrial Dynamics at the Interface of the Inner and Outer Membranes

Abstract : Dynamic interactions between components of the outer (OM) and inner (IM) membranes control a number of critical mitochondrial functions such as channeling of metabolites and coordinated fission and fusion. We identify here the mitochondrial AAA(+) ATPase protein ATAD3A specific to multicellular eukaryotes as a participant in these interactions. The N-terminal domain interacts with the OM. A central transmembrane segment (TMS) anchors the protein in the IM and positions the C-terminal AAA(+) ATPase domain in the matrix. Invalidation studies in Drosophila and in a human steroidogenic cell line showed that ATAD3A is required for normal cell growth and cholesterol channeling at contact sites. Using dominant-negative mutants, including a defective ATP-binding mutant and a truncated 50-amino-acid N-terminus mutant, we showed that ATAD3A regulates dynamic interactions between the mitochondrial OM and IM sensed by the cell fission machinery. The capacity of ATAD3A to impact essential mitochondrial functions and organization suggests that it possesses unique properties in regulating mitochondrial dynamics and cellular functions in multicellular organisms.
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https://hal.univ-grenoble-alpes.fr/hal-02459107
Contributeur : Nadia Cherradi <>
Soumis le : mercredi 29 janvier 2020 - 10:57:56
Dernière modification le : lundi 31 août 2020 - 12:36:19

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Benoit Gilquin, Emmanuel Taillebourg, Nadia Cherradi, Arnaud Hubstenberger, Olivia Gay, et al.. The AAA+ ATPase ATAD3A Controls Mitochondrial Dynamics at the Interface of the Inner and Outer Membranes. Molecular and Cellular Biology, American Society for Microbiology, 2010, 30 (8), pp.1984-1996. ⟨10.1128/MCB.00007-10⟩. ⟨hal-02459107⟩

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