Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells

Abstract : We engineered the first chimeric, bispecific lectin, with two rationally oriented and distinct recognition surfaces. This lectin, coined Janus lectin in allusion to the two-faced roman god, is able to bind independently to both fucosylated and sialylated glycoconjugates. The multivalent presentation of binding sites on each face of the Janus lectin is very efficient, resulting in avidities in the low nanomolar range for both fucosylated and sialylated surfaces. Moreover, novel heterovalent, bifunctional glycoclusters were synthetized that match the topology of the Janus lectin. Based on these tools, we constructed organized and controlled supramolecular architectures by assembling Janus lectin and glycocompound layer-by-layer. Furthermore, the Janus lectin was employed as biomolecular linker to organize protocells made from giant unilamellar vesicles of different nature, to more complex prototissues. In summary, tailor-made Janus lectins open wide possibilities for creating biomimetic matrices or artificial tissues.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [29 références]  Voir  Masquer  Télécharger
Contributeur : David Goyard <>
Soumis le : lundi 1 avril 2019 - 10:58:15
Dernière modification le : mardi 17 novembre 2020 - 12:12:03
Archivage à long terme le : : mardi 2 juillet 2019 - 13:42:13


Fichiers éditeurs autorisés sur une archive ouverte




João-Paulo Ribeiro, Sarah Villringer, David Goyard, Liliane Coche-Guerente, Manuela Höferlin, et al.. Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells. Chemical Science , The Royal Society of Chemistry, 2018, 9 (39), pp.7634-7641. ⟨10.1039/c8sc02730g⟩. ⟨hal-02086152⟩



Consultations de la notice


Téléchargements de fichiers