Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin

Abstract : Lipopolysaccharides (LPS) are complex glycolipids forming the outside layer of Gram-negative bacteria. Their hydrophobic and heterogeneous nature greatly hampers their structural study in an environment similar to the bacterial surface. We have studied LPS purified from E. coli and pathogenic P. aeruginosa with long O-antigen polysaccharides assembled in solution as vesicles or elongated micelles. Solid-state NMR with magic-angle spinning permitted the identification of NMR signals arising from regions with different flexibilities in the LPS, from the lipid components to the O-antigen polysaccharides. Atomic scale data on the LPS enabled the study of the interaction of gentamicin antibiotic bound to P. aeruginosa LPS, for which we could confirm that a specific oligosaccharide is involved in the antibiotic binding. The possibility to study LPS alone and bound to a ligand when it is assembled in membrane-like structures opens great prospects for the investigation of proteins and antibiotics that specifically target such an important molecule at the surface of Gram-negative bacteria.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-grenoble-alpes.fr/hal-01875923
Contributeur : Frank Thomas <>
Soumis le : mardi 18 septembre 2018 - 09:00:53
Dernière modification le : mercredi 15 juillet 2020 - 13:02:04

Identifiants

Collections

Citation

Cédric Laguri, Alba Silipo, Alessandra Martorana, Paul Schanda, Roberta Marchetti, et al.. Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin. ACS Chemical Biology, American Chemical Society, 2018, 13 (8), pp.2106 - 2113. ⟨10.1021/acschembio.8b00271⟩. ⟨hal-01875923⟩

Partager

Métriques

Consultations de la notice

429