Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Three Novel Escherichia coli Vectors for Convenient and Efficient Molecular Biological Manipulations

Abstract : We have constructed novel plasmids pANY2, pANY3, and pANY6 for flexible cloning with low false positives, efficient expression, and convenient purification of proteins. The pANY2 plasmid can be used for efficient isopropyl-β-d-thiogalactoside (IPTG) induced protein expression, while the pANY3 plasmid can be used for temperature-induced expression. The pANY6 plasmid contains a self-cleaving elastin-like protein (ELP) tag for purification of recombinant protein by simple ELP-mediated precipitation steps and removal of the ELP tag by self-cleavage. A urea-based denaturation and refolding processes for renaturation of insoluble inclusion bodies can be conveniently integrated into the ELP-mediated precipitation protocol, removing time-consuming dialysis steps. These novel vectors, together with the described strategies of gene cloning, protein expression, and purification, may have wide applications in biosciences, agricultural, food technologies, and so forth.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-grenoble-alpes.fr/hal-01832799
Contributeur : Frank Thomas <>
Soumis le : lundi 9 juillet 2018 - 09:10:15
Dernière modification le : mardi 6 octobre 2020 - 16:12:09

Identifiants

Collections

Citation

Herui Gao, Xia Qi, Darren J Hart, Song Gao, Hongling Wang, et al.. Three Novel Escherichia coli Vectors for Convenient and Efficient Molecular Biological Manipulations. Journal of Agricultural and Food Chemistry, American Chemical Society, 2018, 66 (24), pp.6123 - 6131. ⟨10.1021/acs.jafc.8b01960⟩. ⟨hal-01832799⟩

Partager

Métriques

Consultations de la notice

313