Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Expression and Characterization of Levansucrase from Clostridium acetobutylicum.

Abstract : The Clostridium acetobutylicum gene Ca-SacB encoding levansucrase was cloned and expressed in Escherichia coli. Ca-SacB is composed of 1287 bp and encodes 428 amino acid residues, which could convert 150 mmol/L sucrose to levan with the liberation of glucose. The optimum pH and temperature of this enzyme for levan formation were pH 6 and 60 °C, respectively. Levansucrase activity of Ca-SacB was completely abolished by 5 mmol/L Ag(+) and Hg(2+). The Km and Vmax values for levansucrase were calculated to be 64 mmol/L and 190 μmol/min/mg, respectively. Interestingly, Ca-SacB was found to have high product specificity, and no fructooligosaccharide was identified in the product, indicating that Ca-SacB may be valuable for industrial production of levan. In addition, Ca-SacB is the first characterized levansucrase isolated from an anaerobic bacterium, which should be valuable for exploring new enzyme resources and deepening the understanding of the catalytic mechanisms of levansucrases.
Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Frank Thomas <>
Soumis le : mercredi 29 mars 2017 - 10:21:51
Dernière modification le : mardi 6 octobre 2020 - 16:12:09




Song Gao, Xianghui Qi, Darren J Hart, Herui Gao, Yingfeng An. Expression and Characterization of Levansucrase from Clostridium acetobutylicum.. Journal of Agricultural and Food Chemistry, American Chemical Society, 2017, 65 (4), pp.867-871. ⟨10.1021/acs.jafc.6b05165⟩. ⟨hal-01497700⟩



Consultations de la notice