A three-dimensional movie of structural changes in bacteriorhodopsin.
Eriko Nango
(1)
,
Antoine Royant
(2, 3)
,
Minoru Kubo
(1)
,
Takanori Nakane
(4)
,
Cecilia Wickstrand
(5)
,
Tetsunari Kimura
(1)
,
Tomoyuki Tanaka
(1)
,
Kensuke Tono
(1)
,
So Song
(1)
,
Rie Tanaka
(1)
,
Toshi Arima
(1)
,
Ayumi Yamashita
(1)
,
Jun Kobayashi
(1)
,
Toshiaki Hosaka
(6)
,
Eiichi Mizohata
(7)
,
Przemyslaw Nogly
(8)
,
Michihiro Sugahara
(1)
,
Daewoong Nam
(9, 10)
,
Takashi Nomura
(1)
,
Tatsuro Shimamura
(11)
,
Dohyun Im
(11)
,
Takaaki Fujiwara
(11)
,
Yasuaki Yamanaka
(11)
,
Byeonghyun Jeon
(10)
,
Tomohiro Nishizawa
(4)
,
Kazumasa Oda
(4)
,
Masahiro Fukuda
(4)
,
Rebecka Andersson
(5)
,
Petra Båth
(5)
,
Robert Dods
(5)
,
Jan Davidsson
(12)
,
Shigeru Matsuoka
(13)
,
Satoshi Kawatake
(13)
,
Michio Murata
(13)
,
Osamu Nureki
(4)
,
Shigeki Owada
(1)
,
Takashi Kameshima
(14)
,
Takaki Hatsui
(1)
,
Yasumasa Joti
(14)
,
Gebhard Schertler
(8)
,
Makina Yabashi
(1)
,
Ana-Nicoleta Bondar
(15)
,
Jörg Standfuss
(8)
,
Richard Neutze
(5)
,
So Iwata
(11)
1
RIKEN -
RIKEN - Institute of Physical and Chemical Research [Japon]
2 IBS - UMR 5075 - Institut de biologie structurale
3 ESRF - European Synchrotron Radiation Facility
4 Department of Biological Sciences
5 Department of Chemistry and Molecular Biology [Gothenburg]
6 Division of Structural and Synthetic Biology
7 Department of Applied Chemistry
8 Division of Biology and Chemistry, Laboratory for Biomolecular Research
9 INRS-IAF - Institut Armand Frappier
10 Department of Physics
11 Department of Cell Biology, Graduate School of Medicine
12 Angström Laboratory
13 JST-Exploratory Research for Advanced Technology (ERATO)
14 JASRI - Japan Synchrotron Radiation Research Institute [Hyogo]
15 Theoretical Molecular Biophysics, Department of Physics
2 IBS - UMR 5075 - Institut de biologie structurale
3 ESRF - European Synchrotron Radiation Facility
4 Department of Biological Sciences
5 Department of Chemistry and Molecular Biology [Gothenburg]
6 Division of Structural and Synthetic Biology
7 Department of Applied Chemistry
8 Division of Biology and Chemistry, Laboratory for Biomolecular Research
9 INRS-IAF - Institut Armand Frappier
10 Department of Physics
11 Department of Cell Biology, Graduate School of Medicine
12 Angström Laboratory
13 JST-Exploratory Research for Advanced Technology (ERATO)
14 JASRI - Japan Synchrotron Radiation Research Institute [Hyogo]
15 Theoretical Molecular Biophysics, Department of Physics
Antoine Royant
- Fonction : Auteur
- PersonId : 1640
- IdHAL : antoine-royant
- ORCID : 0000-0002-1919-8649
- IdRef : 16610020X
Kensuke Tono
- Fonction : Auteur
- PersonId : 780426
- ORCID : 0000-0003-1218-3759
Osamu Nureki
- Fonction : Auteur
- PersonId : 762915
- ORCID : 0000-0003-1813-7008
Shigeki Owada
- Fonction : Auteur
- PersonId : 780427
- ORCID : 0000-0002-1451-7612
Makina Yabashi
- Fonction : Auteur
- PersonId : 763945
- ORCID : 0000-0002-2472-1684
Résumé
Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.