Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Structures of parasite calreticulins provide insights into their flexibility and dual carbohydrate/peptide-binding properties

Abstract : Understanding adaptation to extreme environments remains a challenge of high biotechnological potential for fundamental molecular biology. The cytosol of many microorganisms, isolated from saline environments, reversibly accumulates molar concentrations of the osmolyte ectoine to counterbalance fluctuating external salt concentrations. Although they have been studied extensively by thermodynamic and spectroscopic methods, direct experimental structural data have, so far, been lacking on ectoine-water-protein interactions. In this paper, in vivo deuterium labeling, small angle neutron scattering, neutron membrane diffraction and inelastic scattering are combined with neutron liquids diffraction to characterize the extreme ectoine-containing solvent and its effects on purple membrane of H. salinarum and E. coli maltose binding protein. The data reveal that ectoine is excluded from the hydration layer at the membrane surface and does not affect membrane molecular dynamics, and prove a previous hypothesis that ectoine is excluded from a monolayer of dense hydration water around the soluble protein. Neutron liquids diffraction to atomic resolution shows how ectoine enhances the remarkable properties of H-bonds in water-properties that are essential for the proper organization, stabilization and dynamics of biological structures.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [36 références]  Voir  Masquer  Télécharger
Contributeur : Frank Thomas <>
Soumis le : lundi 28 novembre 2016 - 11:02:59
Dernière modification le : mardi 6 octobre 2020 - 16:12:09
Archivage à long terme le : : lundi 20 mars 2017 - 17:08:26


Fichiers éditeurs autorisés sur une archive ouverte




Christophe J Moreau, Gianluca Cioci, Marina Iannello, Emmanuelle Laffly, Anne Chouquet, et al.. Structures of parasite calreticulins provide insights into their flexibility and dual carbohydrate/peptide-binding properties. IUCrJ = International Union of Crystallography journal, International Union of Crystallography 2016, 3 (6), pp.408-419. ⟨10.1107/S2052252516012847⟩. ⟨hal-01396245⟩



Consultations de la notice


Téléchargements de fichiers