Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence-Dependence. - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Angewandte Chemie International Edition Année : 2016

Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence-Dependence.

Résumé

Measles virus RNA genomes are packaged into helical nucleocapsids (NCs), comprising thousands of nucleo-proteins (N) that bind the entire genome. N-RNA provides the template for replication and transcription by the viral polymerase and is a promising target for viral inhibition. Elucidation of mechanisms regulating this process has been severely hampered by the inability to controllably assemble NCs. Here, we demonstrate self-organization of N into NC-like particles in vitro upon addition of RNA, providing a simple and versatile tool for investigating assembly. Real-time NMR and fluorescence spectroscopy reveals biphasic assembly kinetics. Remarkably, assembly depends strongly on the RNA-sequence, with the genomic 5' end and poly-Adenine sequences assembling efficiently, while sequences such as poly-Uracil are incompetent for NC formation. This observation has important consequences for understanding the assembly process.

Dates et versions

hal-01341976 , version 1 (05-07-2016)

Identifiants

Citer

Sigrid Milles, Malene Ringkjøbing Jensen, Guillaume Communie, Damien Maurin, Guy Schoehn, et al.. Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence-Dependence.. Angewandte Chemie International Edition, 2016, ⟨10.1002/anie.201602619⟩. ⟨hal-01341976⟩
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