A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation - Archive ouverte HAL Access content directly
Journal Articles PLoS ONE Year : 2015

A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

Huaying Zhao (1) , Rodolfo Ghirlando , Carlos Alfonso , Fumio Arisaka , Ilan Attali , David L Bain (2) , Marina M Bakhtina , Donald F Becker (3) , Gregory J Bedwell , Ahmet Bekdemir , Tabot M D Besong , Catherine Birck (4) , Chad A Brautigam , William Brennerman , Olwyn Byron , Agnieszka Bzowska , Jonathan B Chaires , Catherine T Chaton (5) , Helmut Cölfen (6) , Keith D Connaghan , Kimberly A Crowley , Ute Curth , Tina Daviter , William L Dean , Ana I Díez (7) , Christine Ebel (8) , Debra M Eckert (9) , Leslie E Eisele , Edward Eisenstein , Patrick England (10) , Carlos Escalante , Jeffrey A Fagan , Robert Fairman , Ron M Finn , Wolfgang Fischle (11) , José García de La Torre , Jayesh Gor , Henning Gustafsson , Damien Hall (12) , Stephen E Harding (13) , José G Hernández Cifre , Andrew B Herr , Elizabeth E Howell , Richard S Isaac (14) , Shu-Chuan Jao , Davis Jose (15) , Soon-Jong Kim , Bashkim Kokona , Jack A Kornblatt , Dalibor Kosek , Elena Krayukhina , Daniel Krzizike , Eric A Kusznir , Hyewon Kwon (16) , Adam Larson (17) , Thomas M Laue , Aline Le Roy (8) , Andrew P Leech , Hauke Lilie , Karolin Luger , Juan R Luque-Ortega , Jia Ma (18) , Carrie A May , Ernest L Maynard , Anna Modrak-Wojcik , Yee-Foong Mok , Norbert Mücke , Luitgard Nagel-Steger , Geeta J Narlikar , Masanori Noda , Amanda Nourse , Tomas Obsil , Chad K Park (19) , Jin-Ku Park (20) , Peter D Pawelek , Erby E Perdue , Stephen J Perkins (21) , Matthew A Perugini , Craig L Peterson , Martin G Peverelli , Grzegorz Piszczek , Gali Prag , Peter E Prevelige , Bertrand D E Raynal (10, 22) , Lenka Rezabkova , Klaus Richter (23) , Alison E Ringel , Rose Rosenberg (24) , Arthur J Rowe (25) , Arne C Rufer (26) , David J Scott (27) , Javier G Seravalli , Alexandra S Solovyova (28) , Renjie Song (29) , David Staunton , Caitlin Stoddard , Katherine Stott , Holger M Strauss (30) , Werner W Streicher , John P Sumida , Sarah G Swygert , Roman H Szczepanowski , Ingrid Tessmer , Ronald T Toth (31) , Ashutosh Tripathy (32) , Susumu Uchiyama , Stephan F W Uebel , Satoru Unzai , Anna Vitlin Gruber (33) , Peter H von Hippel , Christine Wandrey , Szu-Huan Wang (34) , Steven E Weitzel , Beata Wielgus-Kutrowska , Cynthia Wolberger , Martin Wolff , Edward Wright (35) , Yu-Sung Wu (36) , Jacinta M Wubben , Peter Schuck (37)
1 BGI - Beijing Genomics Institute [Shenzhen]
2 LIESP - Laboratoire d'Informatique pour l'Entreprise et les Systèmes de Production
3 ICL - Innovative Computing Laboratory [Knoxville]
4 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
5 EDF R&D - EDF R&D
6 Physikalische Chemie
7 Photonic Crystal Fibres group
8 IBS - UMR 5075 - Institut de biologie structurale
9 ObsGE - Observatoire Astronomique de l'Université de Genève
10 Biophysique des macromolécules et leurs interactions
11 Laboratory of Chromatin Biochemistry [Göttingen]
12 Stellenbosch University
13 Department of Biochemistry
14 The Centre for Ornithology, School of Biosciences, College of Life and Environmental Sciences
15 DCM - CIRE - Département de Chimie Moléculaire - Chimie Inorganique Redox
16 Hormone Research Center
17 ACE - Anglophonie : Communautés, Ecritures
18 IC2MP - Institut de Chimie des Milieux et Matériaux de Poitiers
19 Department of Animal Science
20 ULaval - Université Laval [Québec]
21 School of Mathematics [Bristol]
22 Chimie et Biocatalyse
23 LPS - Laboratoire de Physique des Solides
24 Department of marine Ecology
25 LPMC - Laboratoire de physique de la matière condensée
26 Pharma Research Discovery Chemistry
27 UKZN - University of KwaZulu-Natal [Durban, Afrique du Sud]
28 Institute for Cell and Molecular Biosciences
29 Changzhou University
30 Institut für Geologie und Paläontologie
31 TU/e - Eindhoven University of Technology [Eindhoven]
32 Department of Biochemistry and Biophysics, UNC Macromolecular Interactions Facility
33 Institute of Botany
34 NSF Center for EUV Science and Technology
35 Sustainable Energy Economics
36 Department of mathematics and MOE-LSC
37 STLO - Science et Technologie du Lait et de l'Oeuf
Rodolfo Ghirlando
  • Function : Author
Carlos Alfonso
  • Function : Author
Fumio Arisaka
  • Function : Author
Ilan Attali
  • Function : Author
Marina M Bakhtina
  • Function : Author
Gregory J Bedwell
  • Function : Author
Ahmet Bekdemir
  • Function : Author
Tabot M D Besong
  • Function : Author
Chad A Brautigam
  • Function : Author
William Brennerman
  • Function : Author
Olwyn Byron
  • Function : Author
Agnieszka Bzowska
  • Function : Author
Jonathan B Chaires
  • Function : Author
Catherine T Chaton
  • Function : Author
Keith D Connaghan
  • Function : Author
Kimberly A Crowley
  • Function : Author
Ute Curth
  • Function : Author
Tina Daviter
  • Function : Author
William L Dean
  • Function : Author
Christine Ebel
Leslie E Eisele
  • Function : Author
Edward Eisenstein
  • Function : Author
Carlos Escalante
  • Function : Author
Jeffrey A Fagan
  • Function : Author
Robert Fairman
  • Function : Author
Ron M Finn
  • Function : Author
José García de La Torre
  • Function : Author
Jayesh Gor
  • Function : Author
Henning Gustafsson
  • Function : Author
José G Hernández Cifre
  • Function : Author
Andrew B Herr
  • Function : Author
Elizabeth E Howell
  • Function : Author
Shu-Chuan Jao
  • Function : Author
Soon-Jong Kim
  • Function : Author
Bashkim Kokona
  • Function : Author
Jack A Kornblatt
  • Function : Author
Dalibor Kosek
  • Function : Author
Elena Krayukhina
  • Function : Author
Daniel Krzizike
  • Function : Author
Eric A Kusznir
  • Function : Author
Thomas M Laue
  • Function : Author
Andrew P Leech
  • Function : Author
Hauke Lilie
  • Function : Author
Karolin Luger
  • Function : Author
Juan R Luque-Ortega
  • Function : Author
Carrie A May
  • Function : Author
Ernest L Maynard
  • Function : Author
Anna Modrak-Wojcik
  • Function : Author
Yee-Foong Mok
  • Function : Author
Norbert Mücke
  • Function : Author
Luitgard Nagel-Steger
  • Function : Author
Geeta J Narlikar
  • Function : Author
Masanori Noda
  • Function : Author
Amanda Nourse
  • Function : Author
Tomas Obsil
  • Function : Author
Peter D Pawelek
  • Function : Author
Erby E Perdue
  • Function : Author
Matthew A Perugini
  • Function : Author
Craig L Peterson
  • Function : Author
Martin G Peverelli
  • Function : Author
Grzegorz Piszczek
Gali Prag
  • Function : Author
Peter E Prevelige
  • Function : Author
Lenka Rezabkova
  • Function : Author
Alison E Ringel
  • Function : Author
Javier G Seravalli
  • Function : Author
Renjie Song
  • Function : Author
David Staunton
  • Function : Author
Caitlin Stoddard
  • Function : Author
Katherine Stott
  • Function : Author
Werner W Streicher
  • Function : Author
John P Sumida
  • Function : Author
Sarah G Swygert
  • Function : Author
Roman H Szczepanowski
  • Function : Author
Ingrid Tessmer
  • Function : Author
Susumu Uchiyama
  • Function : Author
Stephan F W Uebel
  • Function : Author
Satoru Unzai
  • Function : Author
Peter H von Hippel
  • Function : Author
Christine Wandrey
  • Function : Author
Steven E Weitzel
  • Function : Author
Beata Wielgus-Kutrowska
  • Function : Author
Cynthia Wolberger
  • Function : Author
Martin Wolff
  • Function : Author
Jacinta M Wubben
  • Function : Author

Abstract

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304 ± 0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of ± 0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies.

Dates and versions

hal-01199168 , version 1 (15-09-2015)

Identifiers

Cite

Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, et al.. A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. PLoS ONE, 2015, 10 (5), pp.e0126420. ⟨10.1371/journal.pone.0126420⟩. ⟨hal-01199168⟩
1449 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More