Structural virology. Near-atomic cryo-EM structure of the helical measles virus nucleocapsid. - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Science Année : 2015

Structural virology. Near-atomic cryo-EM structure of the helical measles virus nucleocapsid.

Résumé

Measles is a highly contagious human disease. We used cryo-electron microscopy and single particle-based helical image analysis to determine the structure of the helical nucleocapsid formed by the folded domain of the measles virus nucleoprotein encapsidating an RNA at a resolution of 4.3 angstroms. The resulting pseudoatomic model of the measles virus nucleocapsid offers important insights into the mechanism of the helical polymerization of nucleocapsids of negative-strand RNA viruses, in particular via the exchange subdomains of the nucleoprotein. The structure reveals the mode of the nucleoprotein-RNA interaction and explains why each nucleoprotein of measles virus binds six nucleotides, whereas the respiratory syncytial virus nucleoprotein binds seven. It provides a rational basis for further analysis of measles virus replication and transcription, and reveals potential targets for drug design.
Fichier principal
Vignette du fichier
Gutsche et al. Structural biology.pdf (1.51 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt

Dates et versions

hal-01162615 , version 1 (24-11-2020)

Identifiants

  • HAL Id : hal-01162615 , version 1
  • PUBMED : 25883315

Citer

Irina Gutsche, Ambroise Desfosses, Grégory Effantin, Wai-Li Ling, Melina Haupt, et al.. Structural virology. Near-atomic cryo-EM structure of the helical measles virus nucleocapsid.. Science, 2015, 348 (6235), pp.704-7. ⟨hal-01162615⟩
306 Consultations
115 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More