Structural features of the interaction of MapZ with FtsZ and membranes in Streptococcus pneumoniae - Université Grenoble Alpes
Article Dans Une Revue Scientific Reports Année : 2020

Structural features of the interaction of MapZ with FtsZ and membranes in Streptococcus pneumoniae

Résumé

MapZ localizes at midcell and acts as a molecular beacon for the positioning of the cell division machinery in the bacterium Streptococcus pneumoniae. MapZ contains a single transmembrane helix that separates the C-terminal extracellular domain from the N-terminal cytoplasmic domain. Only the structure and function of the extracellular domain is known. Here, we demonstrate that large parts of the cytoplasmic domain is intrinsically disordered and that there are two regions (from residues 45 to 68 and 79 to 95) with a tendency to fold into amphipathic helices. We further reveal that these regions interact with the surface of liposomes that mimic the Streptococcus pneumoniae cell membrane. The highly conserved and unfolded N-terminal region (from residues 17 to 43) specifically interacts with FtsZ independently of FtsZ polymerization state. Moreover, we show that MapZ phosphorylation at positions Thr67 and Thr68 does not impact the interaction with FtsZ or liposomes. Altogether, we propose a model in which the MapZ-mediated recruitment of FtsZ to mid-cell is modulated through competition of MapZ binding to the cell membrane. The molecular interplay between the components of this tripartite complex could represent a key step toward the complete assembly of the divisome.
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hal-01119769 , version 1 (23-11-2020)

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Tomáš Hošek, Catherine M Bougault, Jean-Pierre Lavergne, Denis Martinez, Isabel Ayala, et al.. Structural features of the interaction of MapZ with FtsZ and membranes in Streptococcus pneumoniae. Scientific Reports, 2020, 10 (1), pp.4051. ⟨10.1038/s41598-020-61036-9⟩. ⟨hal-01119769⟩
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