Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon - Groupe Réponse immunitaire aux pathogènes et au soi altéré / Immune Response to Pathogens and altered-self Group (IBS-IRPAS) Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2020

Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

Résumé

Halophilic proteins subjected to below about 15% salt in vitro denature through misfolding, aggregation and/or precipitation. Halobacteria, however, have been detected in environments of fluctuating salinity such as coastal salterns and even around fresh water springs in the depths of the Dead Sea. In order to identify the underlying mechanisms of low salt survival, we explored the reactivation capacity of Halobacterium (Hbt) salinarum sub-populations after incubation in low salt media and recovery in physiological salt. Respiratory oxygen consumption was assessed in stressed cells and cell viability was estimated by Live/Dead staining and flow cytometry. In vivo neutron scattering experiments showed that the recovery of Hbt salinarum sub-populations exposed to severe low salt conditions is related to a rapid retrieval of functional molecular dynamics in the proteome. In the hypothesis that the observations on Hbt salinarum have wider relevance, they could be of key ecological significance for the dispersion of extremophiles when environmental fluctuations become severe.
Fichier principal
Vignette du fichier
Vauclare et al. Scientific Reports.pdf (1.48 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02519472 , version 1 (23-11-2020)

Identifiants

Citer

Pierre Vauclare, F. Natali, Jean-Philippe Kleman, Giuseppe Zaccai, Bruno Franzetti. Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon. Scientific Reports, 2020, 10 (1), pp.3298. ⟨10.1038/s41598-020-59681-1⟩. ⟨hal-02519472⟩
173 Consultations
72 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More