Cell morphology and nucleoid dynamics in dividing Deinococcus radiodurans - Groupe Réponse immunitaire aux pathogènes et au soi altéré / Immune Response to Pathogens and altered-self Group (IBS-IRPAS) Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2019

Cell morphology and nucleoid dynamics in dividing Deinococcus radiodurans

Résumé

Our knowledge of bacterial nucleoids originates mostly from studies of rod-or crescentshaped bacteria. Here we reveal that Deinococcus radiodurans, a relatively large spherical bacterium with a multipartite genome, constitutes a valuable system for the study of the nucleoid in cocci. Using advanced microscopy, we show that D. radiodurans undergoes coordinated morphological changes at both the cellular and nucleoid level as it progresses through its cell cycle. The nucleoid is highly condensed, but also surprisingly dynamic, adopting multiple configurations and presenting an unusual arrangement in which oriC loci are radially distributed around clustered ter sites maintained at the cell centre. Single-particle tracking and fluorescence recovery after photobleaching studies of the histone-like HU protein suggest that its loose binding to DNA may contribute to this remarkable plasticity. These findings demonstrate that nucleoid organization is complex and tightly coupled to cell cycle progression in this organism.
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Dates et versions

hal-02273077 , version 1 (23-11-2020)

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Kevin Floc’h, Françoise Lacroix, Pascale Servant, Yung-Sing Wong, Jean-Philippe Kleman, et al.. Cell morphology and nucleoid dynamics in dividing Deinococcus radiodurans. Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-11725-5⟩. ⟨hal-02273077⟩
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