Cell-free expression of the outer membrane protein OprF of $Pseudomonas\ aeruginosa$ for vaccine purposes - Archive ouverte HAL Access content directly
Journal Articles Life Science Alliance Year : 2021

Cell-free expression of the outer membrane protein OprF of $Pseudomonas\ aeruginosa$ for vaccine purposes

Abstract

$Pseudomonas\ aeruginosa$ is the second-leading cause of nosocomial infections and pneumonia in hospitals. Because of its extraordinary capacity for developing resistance to antibiotics, treating infections by Pseudomonas is becoming a challenge, lengthening hospital stays, and increasing medical costs and mortality. The outer membrane protein OprF is a well-conserved and immunogenic porin playing an important role in quorum sensing and in biofilm formation. Here, we used a bacterial cell-free expression system to reconstitute OprF under its native forms in liposomes and we demonstrated that the resulting OprF proteoliposomes can be used as a fully functional recombinant vaccine against $P.\ aeruginosa$ . Remarkably, we showed that our system promotes the folding of OprF into its active open oligomerized state as well as the formation of mega-pores. Our approach thus represents an easy and efficient way for producing bacterial membrane antigens exposing native epitopes for vaccine purposes.
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Dates and versions

hal-03358640 , version 1 (30-09-2021)

Licence

Attribution - CC BY 4.0

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Géraldine Mayeux, Landry Gayet, Lavinia Liguori, Marine Odier, Donald Martin, et al.. Cell-free expression of the outer membrane protein OprF of $Pseudomonas\ aeruginosa$ for vaccine purposes. Life Science Alliance, 2021, 4 (6), pp.e202000958. ⟨10.26508/lsa.202000958⟩. ⟨hal-03358640⟩
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