Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Nadide Altincekic; Sophie Marianne Korn; Nusrat Shahin Qureshi; Marie Dujardin; Martí Ninot-Pedrosa; Rupert Abele; Marie Jose Abi Saad; Caterina Alfano; Fabio Almeida; Islam Alshamleh; Gisele Cardoso de Amorim; Thomas Anderson; Cristiane Anobom; Chelsea Anorma; Jasleen Kaur Bains; Adriaan Bax; Martin Blackledge; Julius Blechar; Anja Böckmann; Louis Brigandat; Anna Bula; Matthias Bütikofer; Aldo Camacho-Zarco; Teresa Carlomagno; Icaro Putinhon Caruso; Betül Ceylan; Apirat Chaikuad; Feixia Chu; Laura Cole; Marquise Crosby; Vanessa de Jesus; Karthikeyan Dhamotharan; Isabella Felli; Jan Ferner; Yanick Fleischmann; Marie-Laure Fogeron; Nikolaos Fourkiotis; Christin Fuks; Boris Fürtig; Angelo Gallo; Santosh Gande; Juan Atilio Gerez; Dhiman Ghosh; Francisco Gomes-Neto; Oksana Gorbatyuk; Serafima Guseva; Carolin Hacker; Sabine Häfner; Bing Hao; Bruno Hargittay; K. Henzler-Wildman; Jeffrey Hoch; Katharina Hohmann; Marie Hutchison; Kristaps Jaudzems; Katarina Jović; Janina Kaderli; Gints Kalniņš; Iveta Kaņepe; Robert Kirchdoerfer; John Kirkpatrick; Stefan Knapp; Robin Krishnathas; Felicitas Kutz; Susanne Zur Lage; Roderick Lambertz; Andras Lang; Douglas Laurents; Lauriane Lecoq; Verena Linhard; Frank Löhr; Anas Malki; Luiza Mamigonian Bessa; Rachel Martin; Tobias Matzel; Damien Maurin; Seth Mcnutt; Nathane Cunha Mebus-Antunes; Beat Meier; Nathalie Meiser; Miguel Mompeán; Elisa Monaca; Roland Montserret; Laura Mariño Perez; Celine Moser; Claudia Muhle-Goll; Thais Cristtina Neves-Martins; Xiamonin Ni; Brenna Norton-Baker; Roberta Pierattelli; Letizia Pontoriero; Yulia Pustovalova; Oliver Ohlenschläger; Julien Orts; Andrea da Poian; Dennis Pyper; Christian Richter; Roland Riek; Chad Rienstra; Angus Robertson; Anderson Pinheiro; Raffaele Sabbatella; Nicola Salvi; Krishna Saxena; Linda Schulte; Marco Schiavina; Harald Schwalbe; Mara Silber; Marcius da Silva Almeida; Marc Sprague-Piercy; Georgios Spyroulias; Sridhar Sreeramulu; Jan-Niklas Tants; Kaspars Tārs; Felix Torres; Sabrina Töws; Miguel Treviño; Sven Trucks; Aikaterini Tsika; Krisztina Varga; Ying Wang; Marco Weber; Julia Weigand; Christoph Wiedemann; Julia Wirmer-Bartoschek; Maria Alexandra Wirtz Martin; Johannes Zehnder; Martin Hengesbach; Andreas Schlundt; Miguel Á. Treviño

doi:10.3389/fmolb.2021.653148

Journal Articles Frontiers in Molecular Biosciences Year : 2021

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

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Nadide Altincekic

Function : Author

Sophie Marianne Korn

Function : Author

Institute of Biophysical Chemistry, Center for Biomolecular Magnetic Resonance (BMRZ)

Nusrat Shahin Qureshi

Function : Author

Marie Dujardin

Function : Author

Microbiologie moléculaire et biochimie structurale / Molecular Microbiology and Structural Biochemistry

Martí Ninot-Pedrosa

Function : Author

Rupert Abele

Function : Author

Marie Jose Abi Saad

Function : Author

Caterina Alfano

Function : Author

Fabio Almeida

Function : Author

Islam Alshamleh

Function : Author

Gisele Cardoso de Amorim

Function : Author
PersonId : 773492
ORCID : 0000-0003-4348-9515

Thomas Anderson

Function : Author

Cristiane Anobom

Function : Author

Chelsea Anorma

Function : Author

Jasleen Kaur Bains

Function : Author

Adriaan Bax

Function : Author

Martin Blackledge

Function : Author
PersonId : 760553
ORCID : 0000-0003-0935-721X
IdRef : 095872086

Institut de biologie structurale

Julius Blechar

Function : Author

Anja Böckmann

Function : Author
PersonId : 178190
IdHAL : anja-bockmann
ORCID : 0000-0001-8149-7941
IdRef : 156611430

Louis Brigandat

Function : Author

Anna Bula

Function : Author

Matthias Bütikofer

Function : Author

Aldo Camacho-Zarco

Function : Author
PersonId : 802660
ORCID : 0000-0002-0186-8544

Institut de biologie structurale

Teresa Carlomagno

Function : Author

Icaro Putinhon Caruso

Function : Author

Betül Ceylan

Function : Author

Apirat Chaikuad

Function : Author

Feixia Chu

Function : Author

Laura Cole

Function : Author

Marquise Crosby

Function : Author

Vanessa de Jesus

Function : Author

Karthikeyan Dhamotharan

Function : Author

Isabella Felli

Function : Author

Jan Ferner

Function : Author

Yanick Fleischmann

Function : Author

Marie-Laure Fogeron

Function : Author
PersonId : 763310
ORCID : 0000-0002-2146-8740
IdRef : 188711910

Nikolaos Fourkiotis

Function : Author

Christin Fuks

Function : Author

Boris Fürtig

Function : Author

Angelo Gallo

Function : Author

Santosh Gande

Function : Author

Juan Atilio Gerez

Function : Author

Dhiman Ghosh

Function : Author

Francisco Gomes-Neto

Function : Author

Oksana Gorbatyuk

Function : Author

Serafima Guseva

Function : Author
PersonId : 793044
ORCID : 0000-0001-9706-5035

Institut de biologie structurale

Carolin Hacker

Function : Author

Sabine Häfner

Function : Author

Bing Hao

Function : Author

Bruno Hargittay

Function : Author

K. Henzler-Wildman

Function : Author

Jeffrey Hoch

Function : Author

Katharina Hohmann

Function : Author

Marie Hutchison

Function : Author

Kristaps Jaudzems

Function : Author

Katarina Jović

Function : Author

Janina Kaderli

Function : Author

Gints Kalniņš

Function : Author

Iveta Kaņepe

Function : Author

Robert Kirchdoerfer

Function : Author

John Kirkpatrick

Function : Author

Stefan Knapp

Function : Author

Robin Krishnathas

Function : Author

Felicitas Kutz

Function : Author

Susanne Zur Lage

Function : Author

Roderick Lambertz

Function : Author

Andras Lang

Function : Author

Douglas Laurents

Function : Author

Lauriane Lecoq

Function : Author

Verena Linhard

Function : Author

Frank Löhr

Function : Author

Anas Malki

Function : Author
PersonId : 806463
ORCID : 0000-0001-8336-8289

Institut de biologie structurale

Luiza Mamigonian Bessa

Function : Author
PersonId : 806462
ORCID : 0000-0002-1993-3105

Institut de biologie structurale

Rachel Martin

Function : Author

Tobias Matzel

Function : Author

Damien Maurin

Function : Author

Institut de biologie structurale

Seth Mcnutt

Function : Author

Nathane Cunha Mebus-Antunes

Function : Author

Beat Meier

Function : Author
PersonId : 775924
ORCID : 0000-0002-9107-4464
IdRef : 156274450

Nathalie Meiser

Function : Author

Miguel Mompeán

Function : Author

Elisa Monaca

Function : Author

Roland Montserret

Function : Author
PersonId : 764961
ORCID : 0000-0002-2671-5843

Laura Mariño Perez

Function : Author

Institut de biologie structurale

Celine Moser

Function : Author

Claudia Muhle-Goll

Function : Author

Thais Cristtina Neves-Martins

Function : Author

Xiamonin Ni

Function : Author

Brenna Norton-Baker

Function : Author

Roberta Pierattelli

Function : Author

Letizia Pontoriero

Function : Author

Yulia Pustovalova

Function : Author

Oliver Ohlenschläger

Function : Author

Julien Orts

Function : Author

Andrea da Poian

Function : Author

Dennis Pyper

Function : Author

Christian Richter

Function : Author

Roland Riek

Function : Author

Chad Rienstra

Function : Author

Angus Robertson

Function : Author

Anderson Pinheiro

Function : Author

Raffaele Sabbatella

Function : Author

Nicola Salvi

Function : Author
PersonId : 764706
ORCID : 0000-0003-1515-6908
IdRef : 184453941

Institut de biologie structurale

Krishna Saxena

Function : Author

Linda Schulte

Function : Author

Marco Schiavina

Function : Author

Harald Schwalbe

Function : Author

Mara Silber

Function : Author

Marcius da Silva Almeida

Function : Author

Marc Sprague-Piercy

Function : Author

Georgios Spyroulias

Function : Author

Sridhar Sreeramulu

Function : Author

Jan-Niklas Tants

Function : Author

Kaspars Tārs

Function : Author

Felix Torres

Function : Author

Sabrina Töws

Function : Author

Miguel Treviño

Function : Author

Sven Trucks

Function : Author

Aikaterini Tsika

Function : Author

Krisztina Varga

Function : Author

Ying Wang

Function : Author
PersonId : 801499
ORCID : 0000-0003-4904-8227

Marco Weber

Function : Author

Julia Weigand

Function : Author

Christoph Wiedemann

Function : Author

Julia Wirmer-Bartoschek

Function : Author

Maria Alexandra Wirtz Martin

Function : Author

Johannes Zehnder

Function : Author
PersonId : 807507
ORCID : 0000-0003-3165-6080

Martin Hengesbach

Function : Author

Andreas Schlundt

Function : Author

Miguel Á. Treviño

Function : Author

Abstract

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com , we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.

Keywords

COVID-19 NMR spectroscopy SARS-CoV-2 accessory proteins cell-free protein synthesis intrinsically disordered region nonstructural proteins structural proteins

Domains

Structural Biology [q-bio.BM]

Frank Thomas : Connect in order to contact the contributor

https://hal.univ-grenoble-alpes.fr/hal-03251639

Submitted on : Monday, June 7, 2021-11:28:09 AM

Last modification on : Monday, May 15, 2023-6:57:37 PM

Dates and versions

hal-03251639 , version 1 (07-06-2021)

Identifiers

HAL Id : hal-03251639 , version 1
DOI : 10.3389/fmolb.2021.653148
PUBMED : 34041264
PUBMEDCENTRAL : PMC8141814

Cite

Nadide Altincekic, Sophie Marianne Korn, Nusrat Shahin Qureshi, Marie Dujardin, Martí Ninot-Pedrosa, et al.. Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications. Frontiers in Molecular Biosciences, 2021, 8, pp.653148. ⟨10.3389/fmolb.2021.653148⟩. ⟨hal-03251639⟩

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Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

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