Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue European Biophysics Journal Année : 2021

Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA

Résumé

Fep1 is an iron-responsive GATA-type transcriptional repressor present in numerous fungi. The DNA-binding domain of this protein is characterized by the presence of two zinc fingers of the Cys2-Cys2 type and a Cys-X5-Cys-X8-Cys-X2-Cys motif located between the two zinc fingers, that is involved in binding of a [2Fe-2S] cluster. In this work, biophysical characterization of the DNA-binding domain of Pichia pastoris Fep1 and of the complex of the protein with cognate DNA has been undertaken. The results obtained by analytical ultracentrifugation sedimentation velocity, small-angle X-ray scattering and differential scanning calorimetry indicate that Fep1 is a natively unstructured protein that is able to bind DNA forming 1:1 and 2:1 complexes more compact than the individual partners. Complex formation takes place independently of the presence of a stoichiometric [2Fe-2S] cluster, suggesting that the cluster may play a role in recruiting other protein(s) required for regulation of transcription in response to changes in intracellular iron levels.
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Dates et versions

hal-03163020 , version 1 (09-03-2021)

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Adriana Miele, Laura Cervoni, Aline Le Roy, Antimo Cutone, Giovanni Musci, et al.. Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA. European Biophysics Journal, 2021, ⟨10.1007/s00249-020-01489-y⟩. ⟨hal-03163020⟩
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