Direct Detection of Low Molecular Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry
Résumé
The direct Bio-Layer Interferometry (BLI) meas-urementof low molecular weight analytes (less than 200 Da) still represents a challenge particularly whenlow receptordensities are used. Bio-Layer Interferometry (BLI) is a powerful optical tech-nique for label-free, real-time characterization and quantification of biomolecular interactions at interfaces. We demonstrate herein that the quantification of biomolecular recognition is possible by BLI using either 2D-like or3D platforms for an aptamerligandimmobilization. The influence of the aptamer density on the inter-action was evaluated and compared for the two sensors architec-tures. Despite the low molecular weight (LMW)of the analyte, BLI monitoring led to signals that are exploitablefor affinity and kinetic studies even atlow aptamer density. We demonstrate that the immobilization format as well as the aptamer density hasa crucial influence on the determination of the recognition parame-ters.
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