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Article dans une revue

X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking

Abstract : Here, we describe the crystal structures of two distinct isoforms of ligand-free human karyopherin RanBP5 and investigate its global propensity to interact with influenza A virus polymerase. Our results confirm the general architecture and mechanism of the IMB3 karyopherin-β subfamily whilst also highlighting differences with the yeast orthologue Kap121p. Moreover, our results provide insight into the structural flexibility of β-importins in the unbound state. Based on docking of a nuclear localisation sequence, point mutations were designed, which suppress influenza PA-PB1 subcomplex binding to RanBP5 in a binary protein complementation assay.
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https://hal.univ-grenoble-alpes.fr/hal-02555361
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Soumis le : lundi 27 avril 2020 - 11:36:49
Dernière modification le : lundi 12 octobre 2020 - 10:42:02

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Christopher Swale, Bruno da Costa, Laura Sedano, Frederic Garzoni, Andrew Mccarthy, et al.. X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking. Journal of Molecular Biology, Elsevier, 2020, S0022-2836(20)30254-0, ⟨10.1016/j.jmb.2020.03.021⟩. ⟨hal-02555361⟩

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