Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Science Advances Année : 2020

Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly

Résumé

Many viruses are known to form cellular compartments, also called viral factories. Paramyxoviruses, including measles virus, colocalize their proteomic and genomic material in puncta in infected cells. We demonstrate that purified nucleoproteins (N) and phosphoproteins (P) of measles virus form liquid-like membraneless organelles upon mixing in vitro. We identify weak interactions involving intrinsically disordered domains of N and P that are implicated in this process, one of which is essential for phase separation. Fluorescence allows us to follow the modulation of the dynamics of N and P upon droplet formation, while NMR is used to investigate the thermodynamics of this process. RNA colocalizes to droplets, where it triggers assembly of N protomers into nucleocapsid-like particles that encapsidate the RNA. The rate of encapsidation within droplets is enhanced compared to the dilute phase, revealing one of the roles of liquid-liquid phase separation in measles virus replication.
Fichier principal
Vignette du fichier
Guseva et al. Sci Adv.pdf (4.4 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02529008 , version 1 (23-11-2020)

Identifiants

Citer

Serafima Guseva, Sigrid Milles, Malene Ringkjøbing Jensen, Nicola Salvi, Jean-Philippe Kleman, et al.. Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly. Science Advances , 2020, 6 (14), pp.eaaz7095. ⟨10.1126/sciadv.aaz7095⟩. ⟨hal-02529008⟩
202 Consultations
112 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More