Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2020

Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR

Résumé

The [Fe2S2]-RsrR gene transcription regulator senses the redox status in bacteria by modulating DNA binding, while its cluster cycles between +1 and +2 states-only the latter binds DNA. We have previously shown that RsrR can undergo remarkable conformational changes involving a 100° rotation of tryptophan 9 between exposed (Out) and buried (In) states. Here, we have used the chemical modification of Trp9, site-directed mutagenesis, and crystallographic and computational chemical studies to show that (i) the Out and In states correspond to oxidized and reduced RsrR, respectively, (ii) His33 is protonated in the In state due to a change in its pKa caused by cluster reduction, and (iii) Trp9 rotation is conditioned by the response of its dipole moment to environmental electrostatic changes. Our findings illustrate a novel function of protonation resulting from electron transfer.

Dates et versions

hal-02512906 , version 1 (20-03-2020)

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Citer

Jason C. Crack, Patricia Amara, Anne Volbeda, Jean-Marie Mouesca, Roman Rohac, et al.. Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR. Journal of the American Chemical Society, 2020, 142 (11), pp.5104-5116. ⟨10.1021/jacs.9b12250⟩. ⟨hal-02512906⟩
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