Electron Microscopy of Viruses: Techniques to Prepare Viruses and Viral Proteins for Observation by Electron Microscopy☆ - Université Grenoble Alpes Accéder directement au contenu
Chapitre D'ouvrage Année : 2014

Electron Microscopy of Viruses: Techniques to Prepare Viruses and Viral Proteins for Observation by Electron Microscopy☆

Résumé

Because most viruses are too small to be observed by light microscopy, electron microscopy (EM) is the method of choice for the direct visualization of viruses and viral proteins. To survive the high vacuum inside the microscope, biological samples have to be dehydrated and/or fixed. We describe the different kinds of single particle preparations such as negative staining, cryo-EM and metal shadowing as well as several sectioning techniques including CEMOVIS. We also mention image analysis, 3D-reconstruction and combination of the obtained EM data with data from other structural biology techniques. Images of adenovirus particles, adenovirus proteins and adenovirus-infected cells are used as illustrations.
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Dates et versions

hal-01995929 , version 1 (28-01-2019)

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  • HAL Id : hal-01995929 , version 1

Citer

Pascal Fender, Christine Moriscot, Rob W H Ruigrok, Guy Schoehn. Electron Microscopy of Viruses: Techniques to Prepare Viruses and Viral Proteins for Observation by Electron Microscopy☆. Reference Module in Biomedical Science, Elsevier, 2014. ⟨hal-01995929⟩
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