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Article dans une revue

In Vitro/In Vivo Production of tRNA for X-Ray Studies

Abstract : tRNAs occupy a central role in the cellular life, and they are involved in a broad range of biological processes that relies on their interaction with proteins and RNA. Crystallization and structure resolution of tRNA or/and tRNA/partner complexes can yield in valuable information on structural organizations of key elements of cellular machinery. However, crystallization of RNA, is often challenging. Here we review two methods to produce and purify tRNA in quantity and quality to perform X-ray studies.
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Contributeur : Frank Thomas <>
Soumis le : vendredi 25 janvier 2019 - 10:38:49
Dernière modification le : mercredi 14 octobre 2020 - 04:17:20




Clément Dégut, Alexandre Monod, Franck Brachet, Thibaut Crépin, Carine Tisné. In Vitro/In Vivo Production of tRNA for X-Ray Studies. Methods in Molecular Biology, Humana Press/Springer Imprint, 2016, 1320, pp.37-57. ⟨10.1007/978-1-4939-2763-0_4⟩. ⟨hal-01993848⟩



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