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Article dans une revue

Characterization and Production of Protein Complexes by Co-expression in Escherichia coli

Abstract : The functional units within cells are often macromolecular complexes rather than single species. Production of these complexes as assembled homogenous samples is a prerequisite for their biophysical and structural characterization and hence an understanding of their function in molecular terms. Co-expression in Escherichia coli has been used routinely to decipher the subunit composition, assembly, and production of whole protein complexes. Such complexes can then be used to reconstitute protein/nucleic acid complexes in vitro. In this chapter we present protocols for the widely utilized ACEMBL and pET-MCN/pET-MCP vector series which enable the rapid and automated co-expression of protein complexes in Escherichia coli.
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https://hal.univ-grenoble-alpes.fr/hal-01953513
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Soumis le : jeudi 13 décembre 2018 - 09:25:52
Dernière modification le : mercredi 15 juillet 2020 - 09:10:24

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Matthias Haffke, Martin Marek, Martin Pelosse, Marie-Laure Diebold, Uwe Schlattner, et al.. Characterization and Production of Protein Complexes by Co-expression in Escherichia coli. Methods in Molecular Biology, Humana Press/Springer Imprint, 2015, Escherichia coli, 1261, pp.63-89. ⟨10.1007/978-1-4939-2230-7_4⟩. ⟨hal-01953513⟩

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