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The effects of di(2-ethylhexyl)phthalate on rat liver in relation to selenium status

Abstract : This study was performed to determine the hepatotoxicity of di(2‐ethylhexyl)phthalate (DEHP) in relation to selenium status. In 3‐week‐old Sprague‐Dawley rats, selenium deficiency was induced by a ≤0.05 selenium mg/kg. A selenium supplementation group was given 1 mg selenium/kg diet for 5 weeks. Di(2‐ethylhexyl)phthalate‐treated groups received 1000 mg/kg dose by gavage during the last 10 days of the experiment. Histopathology, peroxisome proliferation, catalase (CAT) immunoreactivity and activity and apoptosis were assessed. Activities of antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase (TrxR1)], superoxide dismutase (SOD), and glutathione S‐transferase (GST); aminotransferase, total glutathione (tGSH), and lipid peroxidation (LP) levels were measured. Di(2‐ethylhexyl)phthalate caused cellular disorganization while necrosis and inflammatory cell infiltration were observed in Se‐deficient DEHP group (DEHP/SeD). Catalase activity and immunoreactivity were increased in all DEHP‐treated groups. Glutathione peroxidase 1 and GPx4 activities decreased significantly in DEHP and DEHP/SeD groups, while GST activities decreased in all DEHP‐exposed groups. Thioredoxin reductase activity increased in DEHP and DEHP/SeS, while total SOD activities increased in all DEHP‐treated groups. Lipid peroxidation levels increased significantly in SeD (26%), DEHP (38%) and DEHP/SeD (71%) groups. Selenium supplementation partially ameliorated DEHP‐induced hepatotoxicity; while in DEHP/SeD group, drastic changes in hepatic histopathology and oxidative stress parameters were observed.
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https://hal.univ-grenoble-alpes.fr/hal-01932829
Contributeur : Sarah Hamant <>
Soumis le : vendredi 23 novembre 2018 - 13:27:55
Dernière modification le : mercredi 15 juillet 2020 - 09:10:04

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Pınar Erkekoglu, Naciye Zeybek, Belma Giray, Walid Rachidi, Murat Kızılgün, et al.. The effects of di(2-ethylhexyl)phthalate on rat liver in relation to selenium status. International Journal of Experimental Pathology, Wiley, 2014, 95 (1), pp.64 - 77. ⟨10.1111/iep.12059⟩. ⟨hal-01932829⟩

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