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Article Dans Une Revue Journal of Physical Chemistry B Année : 2013

Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2

Mark van Der Auweraer
  • Fonction : Auteur
Benjamien Moeyaert
  • Fonction : Auteur
Jan Michiels
  • Fonction : Auteur
Hideaki Mizuno
  • Fonction : Auteur
Johan Hofkens
  • Fonction : Auteur
Virgile Adam

Résumé

Green-to-red photoconversion is a reaction that occurs in a limited number of fluorescent proteins and that is currently mechanistically debated. In this contribution, we report on our investigation of the photoconvertible fluorescent protein Dendra2 by employing a combination of pump-probe, up-conversion and single photon timing spectroscopic techniques. Our findings indicate that upon excitation of the neutral green state an excited state proton transfer proceeds with a time constant of 3.4 ps between the neutral green and the anionic green states. In concentrated solution we detected resonance energy transfer (25 ps time constant) between green and red monomers. The time-resolved emission spectra suggest also the formation of a super-red species, first observed for DsRed (a red fluorescent protein from the corallimorph species Discosoma) and consistent with peculiar structural details present in both proteins.
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Dates et versions

hal-01662511 , version 1 (07-02-2024)

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Eduard Fron, Mark van Der Auweraer, Benjamien Moeyaert, Jan Michiels, Hideaki Mizuno, et al.. Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2. Journal of Physical Chemistry B, 2013, 117 (8), pp.2300 - 2313. ⟨10.1021/jp309219m⟩. ⟨hal-01662511⟩
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