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Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2

Abstract : Green-to-red photoconversion is a reaction that occurs in a limited number of fluorescent proteins and that is currently mechanistically debated. In this contribution, we report on our investigation of the photoconvertible fluorescent protein Dendra2 by employing a combination of pump-probe, up-conversion and single photon timing spectroscopic techniques. Our findings indicate that upon excitation of the neutral green state an excited state proton transfer proceeds with a time constant of 3.4 ps between the neutral green and the anionic green states. In concentrated solution we detected resonance energy transfer (25 ps time constant) between green and red monomers. The time-resolved emission spectra suggest also the formation of a super-red species, first observed for DsRed (a red fluorescent protein from the corallimorph species Discosoma) and consistent with peculiar structural details present in both proteins.
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Contributeur : Frank Thomas <>
Soumis le : mercredi 13 décembre 2017 - 11:17:26
Dernière modification le : mardi 6 octobre 2020 - 16:12:09




Eduard Fron, Mark van der Auweraer, Benjamien Moeyaert, Jan Michiels, Hideaki Mizuno, et al.. Revealing the Excited-State Dynamics of the Fluorescent Protein Dendra2. Journal of Physical Chemistry B, American Chemical Society, 2013, 117 (8), pp.2300 - 2313. ⟨10.1021/jp309219m⟩. ⟨hal-01662511⟩



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