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Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

Abstract : The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
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Contributeur : Frank Thomas <>
Soumis le : vendredi 27 mai 2016 - 10:17:46
Dernière modification le : mercredi 14 octobre 2020 - 04:17:20

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Emilie Montellier, Fayçal Boussouar, Sophie Rousseaux, Kai Zhang, Thierry Buchou, et al.. Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.. Genes and Development, Cold Spring Harbor Laboratory Press, 2013, 27 (15), pp.1680-92. ⟨hal-01322388⟩



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