Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B. - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Genes and Development Année : 2013

Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

Fayçal Boussouar
Kai Zhang
François Fenaille
Hitoshi Shiota
  • Fonction : Auteur
Patrick Héry
  • Fonction : Auteur
Mahya Jamshidikia
Hélène Holota
  • Fonction : Auteur
Aurélie Bergon
Fabrice Lopez
Philippe Guardiola
  • Fonction : Auteur
Karin Pernet
  • Fonction : Auteur
Jean Imbert
Carlo Petosa
Minjia Tan
  • Fonction : Auteur
Yingming Zhao
  • Fonction : Auteur
Matthieu Gérard
  • Fonction : Auteur

Résumé

The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.

Dates et versions

hal-01322388 , version 1 (27-05-2016)

Identifiants

Citer

Emilie Montellier, Fayçal Boussouar, Sophie Rousseaux, Kai Zhang, Thierry Buchou, et al.. Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.. Genes and Development, 2013, 27 (15), pp.1680-92. ⟨hal-01322388⟩
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