Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation - Groupe Entrée et bourgeonnement des virus à enveloppe / Entry and Budding of Enveloped Viruses Group (EBEV) Accéder directement au contenu
Article Dans Une Revue Nature Communications Année : 2020

Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation

Résumé

Endosomal sorting complexes for transport-III (ESCRT-III) assemble in vivo onto membranes with negative Gaussian curvature. How membrane shape influences ESCRT-III polymerization and how ESCRT-III shapes membranes is yet unclear. Human core ESCRT-III proteins, CHMP4B, CHMP2A, CHMP2B and CHMP3 are used to address this issue in vitro by combining membrane nanotube pulling experiments, cryo-electron tomography and AFM. We show that CHMP4B filaments preferentially bind to flat membranes or to tubes with positive mean curvature. Both CHMP2B and CHMP2A/CHMP3 assemble on positively curved membrane tubes. Combinations of CHMP4B/CHMP2B and CHMP4B/CHMP2A/CHMP3 are recruited to the neck of pulled membrane tubes and reshape vesicles into helical "corkscrew-like" membrane tubes. Sub-tomogram averaging reveals that the ESCRT-III filaments assemble parallel and locally perpendicular to the tube axis, highlighting the mechanical stresses imposed by ESCRT-III. Our results underline the versatile membrane remodeling activity of ESCRT-III that may be a general feature required for cellular membrane remodeling processes.
Fichier principal
Vignette du fichier
bertin2020natcom.pdf (3.99 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02651230 , version 1 (29-05-2020)

Identifiants

Citer

Aurélie Bertin, Nicola de Franceschi, Eugenio de La Mora, Sourav Maiti, Maryam Alqabandi, et al.. Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation. Nature Communications, 2020, ⟨10.1038/s41467-020-16368-5⟩. ⟨hal-02651230⟩
130 Consultations
38 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More