S. De-se, La bioluminescence de chaque puits est ensuite lue avec un lecteur de plaque

®. Spectramax and . Xs, Etats-Unis) à 542 nm. (b) Par absorbance

, Après 2 heures d'incubation des cellules avec le sérum humain dilué, puis un lavage au DMEM nonsupplémenté, 110 µL d'une solution de MTT diluée 10 fois (0,5 mg/mL) est ajoutée à chaque puits. Les cristaux de formazan se forment après 1 à 4 heures d'incubation à 37°C et 5 % de CO 2 (dépendant du nombre et du type de cellules). Le milieu de culture est enlevé à la pipette et les cristaux sont dissous dans 100 µL de DMSO pendant 30 minutes à 37°C, De la même manière que pour l'évaluation de la viabilité cellulaire par bioluminescence, les cellules sont détachées et comptées pour laisser adhérer 50 000 cellules/100 µL/puits, mais cette fois-ci dans une microplaque à fond transparent. Les cellules sont traitées comme décrit précédemment, jusqu'à la révélation

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