Embracing Nature’s Wisdom: Liposome-Mediated Amplification of Electrochemiluminescence for the Sensitive and Selective Immunoassay of Serum Amyloid A
Résumé
Serum amyloid A (SAA) is a critical biomarker for diagnosing inflammatory responses in conditions like influenza and COVID-19. To enhance the detection of SAA, we developed an electrochemiluminescence (ECL) biosensor by encapsulating 4,4',4'',4'''-(1,3,6,8-pyrenetetrayl)tetrakis-benzoic acid (TBAPy) within liposomes. This biomimetic encapsulation mimics the protective environment of natural biological membranes, which not only prevents the aggregation of TBAPy but also shields it from quenching effects, thereby significantly increasing the stability and intensity of the ECL signal. The result is a fivefold enhancement in ECL signal intensity with an RSD of 1.71%. The biosensor demonstrated high specificity and sensitivity for SAA detection, with a detection limit of 0.188 pg/mL. This innovative approach, integrating biomimicry with ECL technology, represents a significant advancement in developing next-generation diagnostic tools with superior sensitivity and specificity.
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