NLRP7 Enhances Choriocarcinoma Cell Survival and Camouflage in an Inflammasome Independent Pathway - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Cells Année : 2023

NLRP7 Enhances Choriocarcinoma Cell Survival and Camouflage in an Inflammasome Independent Pathway

Déborah Reynaud
  • Fonction : Auteur
Nadia Alfaidy
Constance Collet
  • Fonction : Auteur
Nicolas Lemaitre
Frederic Sergent
  • Fonction : Auteur
Céline Miege
  • Fonction : Auteur
Emmanuelle Soleilhac
  • Fonction : Auteur
Alaa Al Assi
  • Fonction : Auteur
Padma Murthi
Gilles Courtois
  • Fonction : Auteur
Marie-Odile Fauvarque
  • Fonction : Auteur
Rima Slim
Mohamed Benharouga

Résumé

Background: Gestational choriocarcinoma (GC) is a highly malignant trophoblastic tumor that often develops from a complete hydatidiform mole (HM). NLRP7 is the major gene responsible for recurrent HM and is involved in the innate immune response, inflammation and apoptosis. NLRP7 can function in an inflammasome-dependent or -independent pathway. Recently, we have demonstrated that NLRP7 is highly expressed in GC tumor cells and contributes to their tumorigenesis. However, the underlying mechanisms are still unknown. Here, we investigated the mechanism by which NLRP7 controls these processes in malignant (JEG-3) and non-tumor (HTR8/SVneo) trophoblastic cells. Cell survival, dedifferentiation, camouflage, and aggressiveness were compared between normal JEG-3 cells or knockdown for NLRP7, JEG-3 Sh NLRP7. In addition, HTR8/SVneo cells overexpressing NLRP7 were used to determine the impact of NLRP7 overexpression on non-tumor cells. NLRP7 involvement in tumor cell growth and tolerance was further characterized in vivo using the metastatic mouse model of GC. Results: We demonstrate that NLRP7 (i) functions in an inflammasome-dependent and -independent manners in HTR8/SVneo and JEG-3 cells, respectively; (ii) differentially regulates the activity of NF-κB in tumor and non-tumor cells; (iii) increases malignant cell survival, dedifferentiation, and camouflage; and (iv) facilitates tumor cells colonization of the lungs in the preclinical model of GC. Conclusions: This study demonstrates for the first time the mechanism by which NLRP7, independently of its inflammasome machinery, contributes to GC growth and tumorigenesis. The clinical relevance of NLRP7 in this rare cancer highlights its potential therapeutic promise as a molecular target to treat resistant GC patients.

Dates et versions

hal-04548659 , version 1 (16-04-2024)

Identifiants

Citer

Déborah Reynaud, Nadia Alfaidy, Constance Collet, Nicolas Lemaitre, Frederic Sergent, et al.. NLRP7 Enhances Choriocarcinoma Cell Survival and Camouflage in an Inflammasome Independent Pathway. Cells, 2023, 12 (6), pp.857. ⟨10.3390/cells12060857⟩. ⟨hal-04548659⟩

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