How does $Bacillus\ thuringiensis$ crystallize such a large diversity of toxins? - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Toxins Année : 2021

How does $Bacillus\ thuringiensis$ crystallize such a large diversity of toxins?


Bacillus thuringiensis (Bt) is a natural crystal-making bacterium. Bt diversified into many subspecies that have evolved to produce crystals of hundreds of pesticidal proteins with radically different structures. Their crystalline form ensures stability and controlled release of these major virulence factors. They are responsible for the toxicity and host specificity of Bt, explaining its worldwide use as a biological insecticide. Most research has been devoted to understanding the mechanisms of toxicity of these toxins while the features driving their crystallization have long remained elusive, essentially due to technical limitations. The evolution of methods in structural biology, pushing back the limits in size of amenable protein crystals now allows access to be gained to structural information hidden within natural crystals of such toxins. In this review, we present the main parameters that have been identified as key drivers of toxin crystallization in Bt, notably in the light of recent discoveries driven by structural biology studies. Then, we develop how the future evolution of structural biology will hopefully unveil new mechanisms of Bt toxin crystallization, opening the door to their hijacking with the aim of developing a versatile in vivo crystallization platform of high academic and industrial interest.
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hal-03430907 , version 1 (17-11-2021)




Guillaume Tetreau, Elena Andreeva, Anne-Sophie Banneville, Elke de Zitter, Jacques-Philippe Colletier. How does $Bacillus\ thuringiensis$ crystallize such a large diversity of toxins?. Toxins, 2021, 13 (7), pp.443. ⟨10.3390/toxins13070443⟩. ⟨hal-03430907⟩
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