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Optimizing Chitin Depolymerization by Lysozyme to Long-Chain Oligosaccharides

Abstract : Chitin oligosaccharides (COs) hold high promise as organic fertilizers in the ongoing agro-ecological transition. Short- and long-chain COs can contribute to the establishment of symbiotic associations between plants and microorganisms, facilitating the uptake of soil nutrients by host plants. Long-chain COs trigger plant innate immunity. A fine investigation of these different signaling pathways requires improving the access to high-purity COs. Here, we used the response surface methodology to optimize the production of COs by enzymatic hydrolysis of water-soluble chitin (WSC) with hen egg-white lysozyme. The influence of WSC concentration, its acetylation degree, and the reaction time course were modelled using a Box–Behnken design. Under optimized conditions, water-soluble COs up to the nonasaccharide were formed in 51% yield and purified to homogeneity. This straightforward approach opens new avenues to determine the complex roles of COs in plants.
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Soumis le : mercredi 13 octobre 2021 - 19:09:31
Dernière modification le : vendredi 15 octobre 2021 - 03:06:06

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2021_Masselin_Mar_Drugs.pdf
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Arnaud Masselin, Antoine Rousseau, Stéphanie Pradeau, Laure Fort, Rodolphe Gueret, et al.. Optimizing Chitin Depolymerization by Lysozyme to Long-Chain Oligosaccharides. Marine drugs, MDPI, 2021, 19 (6), pp.320. ⟨10.3390/md19060320⟩. ⟨hal-03247429⟩

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