Anti-Ficolin-2 and Anti-Ficolin-3 Autoantibody Detection by ELISA - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Methods in Molecular Biology Année : 2021

Anti-Ficolin-2 and Anti-Ficolin-3 Autoantibody Detection by ELISA

Résumé

Ficolins are recognition proteins of the lectin pathway of the complement system and also play an important role in innate immunity and in the maintenance of tissue homeostasis. They deserve special attention in the context of autoimmunity since they are involved in the uptake of dying cells. Because the monitoring of systemic lupus erythematosus (SLE) patients is particularly difficult, it is crucial to find new relevant serum biomarkers. The ability to detect autoantibodies in the patients' sera provides a diagnostic and prognostic advantage. We describe in this chapter quantitative enzyme linked immunosorbent assays (ELISA) to detect the presence of autoantibodies targeting ficolin-2 and ficolin-3 in human sera. Recombinant ficolins produced in a mammalian expression system are used as coating antigens. The described in-house ELISAs provide a valuable tool to efficiently quantify anti-ficolin autoantibodies in the sera of SLE patients.
Fichier principal
Vignette du fichier
Dumestrer et al. Methods in Biol Mol.pdf (501.99 Ko) Télécharger le fichier
Origine Accord explicite pour ce dépôt

Dates et versions

hal-03200976 , version 1 (17-04-2021)

Identifiants

Citer

Chantal Dumestre-Pérard, Nicole M. Thielens. Anti-Ficolin-2 and Anti-Ficolin-3 Autoantibody Detection by ELISA. Methods in Molecular Biology, 2021, 2227, pp.121-132. ⟨10.1007/978-1-0716-1016-9_12⟩. ⟨hal-03200976⟩
918 Consultations
277 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More