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Article Dans Une Revue Journal of Immunological Methods Année : 2021

Functional recombinant human complement C1q with different affinity tags

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Résumé

Complement C1q is a multifunctional protein able to sense pathogens and immune molecules such as immunoglobulins and pentraxins, and to trigger the classical complement pathway through activation of its two associated proteases, C1r and C1s. C1q is a multimeric protein composed of three homologous yet distinct polypeptide chains A, B, and C, each composed of an N-terminal collagen-like sequence and a C-terminal globular gC1q module, that assemble into six heterotrimeric (A-B-C) subunits. This hexameric structure exhibits the characteristic shape of a bouquet of flowers, comprising six collagen-like triple helices, each terminating in a trimeric C-terminal globular head. We have produced previously functional recombinant full-length C1q in stably transfected HEK 293-F cells, with a FLAG tag inserted at the C-terminal end of C1qC chain. We report here the generation of additional recombinant C1q proteins, with a FLAG tag fused to the C-terminus of C1qA or C1qB chains, or to the N-terminus of the C1qC chain. Two other variants harboring a Myc or a 6-His tag at the C-terminal end of C1qC were also produced. We show that all C1q variants, except for the His-tagged protein, can be produced at comparable yields and are able to bind with similar affinities to either IgM, a ligand of the globular regions, or to the C1r2-C1s2 tetramer, and to trigger IgM-mediated serum complement activation. These new recombinant C1q variants provide additional tools to investigate the multiple functions of C1q.
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Dates et versions

hal-03152614 , version 1 (25-02-2021)

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Isabelle Bally, Sarah Ancelet, Jean-Baptiste Reiser, Véronique Rossi, Christine Gaboriaud, et al.. Functional recombinant human complement C1q with different affinity tags. Journal of Immunological Methods, 2021, 492, pp.113001. ⟨10.1016/j.jim.2021.113001⟩. ⟨hal-03152614⟩
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