Heart auscultation is one of the most useful medical diagnostic tools for getting valuable information of heart valves and heart hemodynamics functions. However, the information acquired by a traditional stethoscope can be inaccurate and insufficient. Phonocardiogram (PCG) was developed to improve accuracy through visual inspection and analysis. Digitally processed, PCG can then be analyzed by automated heart sound analysis systems. But there is no standardization for PCG data acquisition unlike electrocardiogram (ECG). This study aims at analyzing the influence of cardiomicrophone localization on the chest for the study of cardiac sounds S1 and S2. For that purpose, simultaneous acquisitions of 12 PCG signals with one ECG signal were realized and a comparative analysis of delays between R waves of ECG and detected S1 and S2 sounds was conducted. Results show that there are significant differences between R-S1 (or R-S2) intervals obtained from different areas of sensor placement on the chest. For future works on PCG, studies dealing with the analysis of heart sounds or proposing new heart sounds detection algorithms may pay attention to the location and attachment of PCG sensors.