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Proteomic Identification of Allergenic Proteins of Morus alba L. Pollen

Abstract : BACKGROUND: Tree pollens are well-known aeroallergens all over the world. Little is known about the allergenicity of Morus alba (white mulberry) pollen. OBJECIVE: We aimed to explore the potential allergens of this pollen and its clinical relevance in tree pollen allergic patients living in Istanbul, Turkey. METHODS: Twenty three seasonal allergic rhinitis patients with a confirmed tree pollen allergy and 5 healthy control subjects underwent skin prick and nasal provocation tests with M.alba pollen extract. The pollen extract was then resolved by gel electrophoresis, and immunoblotted with sera from patients/control individuals to detect the potential allergenic proteins. The prevalent IgE binding proteins from 1D-gel were analyzed by MALDI-TOF/TOF. RESULTS: Eleven out of 23 patients were reactive to the extract with skin prick tests. Seven of those patients also reacted positively to the nasal provocation tests. The most common IgE-binding pollen proteins were detected between 55-100 kDa, and also at molecular weights lower than 30 kDa for some patients. Mass spectrometry analyses revealed that the principal IgE-binding protein was methionine synthase (5-methyltetrahydropteroyltriglutamate homocysteine methyltransferase), which is then proposed as a novel allergen in M.alba pollen. CONCLUSION: This study provides the first detailed information for the potential allergens of Morus alba pollen of Istanbul. Methionine synthase with an apparent molecular weight of 80 to 85 kDa has been recognized as one of the allergens in Morus alba pollen for the first time.
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https://hal.univ-grenoble-alpes.fr/hal-01987956
Contributeur : Sarah Hamant <>
Soumis le : lundi 21 janvier 2019 - 14:15:38
Dernière modification le : mardi 28 juillet 2020 - 03:02:28

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  • HAL Id : hal-01987956, version 1

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Çetereisi D, Karlioğlu N, Gelincik A, Sylvie Michelland, Önay-Uçar C, et al.. Proteomic Identification of Allergenic Proteins of Morus alba L. Pollen. Asian Pacific journal of allergy and immunology, Allergy and Immunology Society of Thailand, 2019. ⟨hal-01987956⟩

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