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Article dans une revue

Functional expression of arginine kinase impairs recovery from pH stress of Escherichia coli

Abstract : Acid stress in Escherichia coli involves a complex resource- and energy-consuming response mechanism. By overexpression of arginine kinase from Limulus polyphemus in E. coli, we improved the recovery from a transient pH stress. While wild type E. coli resumed growth after a transient pH reduction to pH 3 for 1 h with a rate that was 25% lower than before the stress, the arginine kinase expressing strain continued to grow as rapidly as before. This effect is presumably caused by the physiological function of arginine kinase as a short term energy buffer in the form of phosphoarginine, but a pH-buffering effect cannot be excluded.
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https://hal.univ-grenoble-alpes.fr/hal-01987873
Contributeur : Sarah Hamant <>
Soumis le : lundi 21 janvier 2019 - 13:19:03
Dernière modification le : mercredi 15 juillet 2020 - 09:10:04

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Canocaco F., Uwe Schlattner, Wallimann T, Sauer U. Functional expression of arginine kinase impairs recovery from pH stress of Escherichia coli. Biotechnology Letters, Springer Verlag, 2003, 25, pp.1013-1017. ⟨10.1023/A:1024172518062⟩. ⟨hal-01987873⟩

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