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Article dans une revue

Delay of airway epithelial wound repair in COPD is associated with airflow obstruction severity

Abstract : BACKGROUND: Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodeling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD. METHODS: Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodeling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. We determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.
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https://hal.univ-grenoble-alpes.fr/hal-01969370
Contributeur : Sarah Hamant <>
Soumis le : vendredi 4 janvier 2019 - 09:25:45
Dernière modification le : mercredi 15 juillet 2020 - 09:10:04

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Jeanne-Marie Perotin, Damien Adam, Juliette Vella-Boucaud, Gonzague Delepine, Sebastian Sandu, et al.. Delay of airway epithelial wound repair in COPD is associated with airflow obstruction severity. Respiratory Research, BioMed Central, 2014, 15 (1), ⟨10.1186/s12931-014-0151-9⟩. ⟨hal-01969370⟩

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