Accéder directement au contenu Accéder directement à la navigation

Application of FRET Biosensors in Energy Metabolism

Abstract : Genetically encoded optical biosensors become a tool of choice for quantitative studies on distribution and concentration changes of ions and metabolites in living cells. In systems biology, they are expected to provide multi-scale analysis in space and time for an advanced understanding of both normal and diseased physiological states. Here we develop and apply fluorescent biosensors based on fluorescence resonance energy transfer (FRET) that are able to monitor directly or indirectly the cellular energy state. Such genetically encoded FRET sensors allow quantitative analysis of changes in adenylate pools or activation of signaling pathways triggered by such changes. This should yield new insight into the spatiotemporal organization of cellular energy metabolism.
Liste complète des métadonnées
Contributeur : Sarah Hamant <>
Soumis le : vendredi 14 décembre 2018 - 11:03:21
Dernière modification le : mercredi 15 juillet 2020 - 09:10:04

Lien texte intégral





Martin Pelosse, Hiromi Imamura, Imre Berger, Uwe Schlattner. Application of FRET Biosensors in Energy Metabolism. 57th Annual Congress of the Biophysical Society, Feb 2013, Philadelphia, United States. Elsevier, 104 (2 suppl 1), pp.304a, 2013, Biophysical Journal. ⟨10.1016/j.bpj.2012.11.1692⟩. ⟨hal-01955201⟩



Consultations de la notice