Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Crystal Structure of Escherichia coli-Expressed Haloarcula marismortui Bacteriorhodopsin I in the Trimeric Form

Abstract : Bacteriorhodopsins are a large family of seven-helical transmembrane proteins that function as light-driven proton pumps. Here, we present the crystal structure of a new member of the family, Haloarcula marismortui bacteriorhodopsin I (HmBRI) D94N mutant, at the resolution of 2.5 angstrom. While the HmBRI retinal-binding pocket and proton donor site are similar to those of other archaeal proton pumps, its proton release region is extended and contains additional water molecules. The protein's fold is reinforced by three novel inter-helical hydrogen bonds, two of which result from double substitutions relative to Halobacterium salinarum bacteriorhodopsin and other similar proteins. Despite the expression in Escherichia coli and consequent absence of native lipids, the protein assembles as a trimer in crystals. The unique extended loop between the helices D and E of HmBRI makes contacts with the adjacent protomer and appears to stabilize the interface. Many lipidic hydrophobic tail groups are discernible in the membrane region, and their positions are similar to those of archaeal isoprenoid lipids in the crystals of other proton pumps, isolated from native or native-like sources. All these features might explain the HmBRI properties and establish the protein as a novel model for the microbial rhodopsin proton pumping studies
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [47 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01573009
Contributeur : Colette Orange <>
Soumis le : mardi 8 août 2017 - 12:02:35
Dernière modification le : mercredi 15 juillet 2020 - 13:02:04

Fichier

Plos_One_Vol_9_pp_e112873.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Vitaly Shevchenko, Ivan Gushchin, Vitaly Polovinkin, Ekaterina Round, Valentin Borshchevskiy, et al.. Crystal Structure of Escherichia coli-Expressed Haloarcula marismortui Bacteriorhodopsin I in the Trimeric Form. PLoS ONE, Public Library of Science, 2014, 9 (12), 20 p. ⟨10.1371/journal.pone.0112873⟩. ⟨hal-01573009⟩

Partager

Métriques

Consultations de la notice

100

Téléchargements de fichiers

233