Highly sensitive and selective detection of L-tryptophan by ECL using boron-doped diamond electrode
Résumé
We report here on the detection of L-Tryptophan, an essential amino-acid involved in neurological functions, using co-reactant-less electrochemiluminescence on boron doped diamond (BDD) electrode. The detection takes place through the unique production of reactive oxygen species (ROS) at the surface of the BDD electrode, which interacts with the oxidized form of L-Tryptophan to produce a luminescent intermediate species. The results show a linear trend between L-Tryptophan concentration and ECL emission peak height, with a correlation co-efficient of R2 = 0.99 in the range from 0-5 µM L-tryptophan. The LOD of this method was found to be 0.77 nM. Comparing to previous work on detecting L-tryptophan these performances are highly promising. Non-ECL techniques such as HPLC (fluorescence detector), or HPLC- MS/MS achieved one level of magnitude higher in sensitivity, in the range of 10-25 nM.
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