Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe-2S] Cluster Coordinated by Cys, Glu and His Residues. - Université Grenoble Alpes Accéder directement au contenu
Article Dans Une Revue Journal of the American Chemical Society Année : 2019

Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe-2S] Cluster Coordinated by Cys, Glu and His Residues.

Ma Teresa Pellicer Martinez
  • Fonction : Auteur
Jason Crack
  • Fonction : Auteur
Patricia Amara
John Munnoch
  • Fonction : Auteur
Matthew Hutchings
  • Fonction : Auteur
Nick Le Brun
  • Fonction : Auteur
  • PersonId : 870227

Résumé

The recently discovered Rrf2 family transcriptional regulator RsrR coordinates a [2Fe-2S] cluster. Remarkably, binding of the protein to RsrR-regulated promoter DNA sequences is switched on and off through the facile cycling of the [2Fe-2S] cluster be-tween +2 and +1 states. Here, we report high resolution crystal structures of the RsrR dimer, revealing that the [2Fe-2S] cluster is asymmetrically coordinated across the RsrR monomer-monomer interface by two Cys residues from one subunit and His and Glu residues from the other. To our knowledge, this is the first example of a protein bound [Fe-S] cluster with three different amino acid side chains as ligands, and of Glu acting as ligand to a [2Fe-2S] cluster. Analyses of RsrR structures revealed a conformation-al change, centered on Trp9, which results in a significant shift in the DNA-binding helix-turn-helix region.

Dates et versions

hal-01991812 , version 1 (24-01-2019)

Identifiants

Citer

Anne Volbeda, Ma Teresa Pellicer Martinez, Jason Crack, Patricia Amara, Océane Gigarel, et al.. Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe-2S] Cluster Coordinated by Cys, Glu and His Residues.. Journal of the American Chemical Society, 2019, 141 (6), pp.2367-2375. ⟨10.1021/jacs.8b10823⟩. ⟨hal-01991812⟩
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