Isolation and characterization of an anti-leishmanial disintegrin from Cerastes cerastes venom

Dihia Allane Habiba Oussedik-Oumehdi Zoubir Harrat Michel Seve 1 Fatima Laraba-Djebari
1 LBFA - Laboratoire de bioénergétique fondamentale et appliquée
Abstract : Investigating new antimicrobial and antiparasitic components from Viperidae venoms represents an alternative therapeutic strategy. In this study, we report the characterization of a disintegrin isolated from Cerastes cerastes venom, exhibiting antiparasitic activity on Leishmania infantum promastigotes. Indeed, isolated disintegrin, referred to Disintegrin_Cc, induced 84.75% of parasiticidal activity and deep morphological alterations on the parasites. SDS-PAGE analysis indicated that this disintegrin was homogenous. This dimeric disintegrin of 14,193.97 Da contains an RGD domain and four intramolecular disulfide bridges. It presents a high percentage of identity with other related snake disintegrins. Predicted 3D structure indicated that this peptide shares partial homology with well-known active antimicrobial peptides. Disintegrin_Cc inhibited 80% of arachidonic acid-induced platelet aggregation. The obtained results suggest that the isolated molecule plays a dual role as a disintegrin and as an anti-leishmanial compound. This component could be useful as a drug in the treatment of leishmaniasis.
Keywords : Cerastes cerastes venom Leishmania infantum disintegrin mass spectrometry
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Journal articles
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http://hal.univ-grenoble-alpes.fr/hal-01989349
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Submitted on : Tuesday, January 22, 2019 - 1:41:07 PM
Last modification on : Thursday, July 18, 2019 - 8:30:02 PM

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Dihia Allane, Habiba Oussedik-Oumehdi, Zoubir Harrat, Michel Seve, Fatima Laraba-Djebari. Isolation and characterization of an anti-leishmanial disintegrin from Cerastes cerastes venom. Journal of Biochemical and Molecular Toxicology, Wiley, 2018, 32 (2), pp.e22018. ⟨10.1002/jbt.22018⟩. ⟨hal-01989349⟩

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