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Isolation and characterization of an anti-leishmanial disintegrin from Cerastes cerastes venom

Abstract : Investigating new antimicrobial and antiparasitic components from Viperidae venoms represents an alternative therapeutic strategy. In this study, we report the characterization of a disintegrin isolated from Cerastes cerastes venom, exhibiting antiparasitic activity on Leishmania infantum promastigotes. Indeed, isolated disintegrin, referred to Disintegrin_Cc, induced 84.75% of parasiticidal activity and deep morphological alterations on the parasites. SDS-PAGE analysis indicated that this disintegrin was homogenous. This dimeric disintegrin of 14,193.97 Da contains an RGD domain and four intramolecular disulfide bridges. It presents a high percentage of identity with other related snake disintegrins. Predicted 3D structure indicated that this peptide shares partial homology with well-known active antimicrobial peptides. Disintegrin_Cc inhibited 80% of arachidonic acid-induced platelet aggregation. The obtained results suggest that the isolated molecule plays a dual role as a disintegrin and as an anti-leishmanial compound. This component could be useful as a drug in the treatment of leishmaniasis.
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http://hal.univ-grenoble-alpes.fr/hal-01989349
Contributeur : Sarah Hamant <>
Soumis le : mardi 22 janvier 2019 - 13:41:07
Dernière modification le : jeudi 11 juin 2020 - 16:30:03

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Dihia Allane, Habiba Oussedik-Oumehdi, Zoubir Harrat, Michel Seve, Fatima Laraba-Djebari. Isolation and characterization of an anti-leishmanial disintegrin from Cerastes cerastes venom. Journal of Biochemical and Molecular Toxicology, Wiley, 2018, 32 (2), pp.e22018. ⟨10.1002/jbt.22018⟩. ⟨hal-01989349⟩

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