ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.

Abstract : Production of soluble, purifiable domains or multi-domain fragments of proteins is a prerequisite for structural biology and other applications. When target sequences are poorly annotated, or when there are few similar sequences available for alignments, identification of domains can be problematic. A method called expression of soluble proteins by random incremental truncation (ESPRIT) addresses this problem by high-throughput automated screening of tens of thousands of enzymatically truncated gene fragments. Rare soluble constructs are identified by experimental screening, and the boundaries revealed by DNA sequencing.
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Methods in Molecular Biology, Humana Press/Springer Imprint, 2017, 1586, pp.45-63. 〈10.1007/978-1-4939-6887-9_4〉
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http://hal.univ-grenoble-alpes.fr/hal-01561714
Contributeur : Frank Thomas <>
Soumis le : jeudi 13 juillet 2017 - 11:29:45
Dernière modification le : lundi 2 juillet 2018 - 16:26:04

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Philippe J Mas, Darren J Hart. ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2017, 1586, pp.45-63. 〈10.1007/978-1-4939-6887-9_4〉. 〈hal-01561714〉

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