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Article Dans Une Revue Methods in Molecular Biology Année : 2017

ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.

Résumé

Production of soluble, purifiable domains or multi-domain fragments of proteins is a prerequisite for structural biology and other applications. When target sequences are poorly annotated, or when there are few similar sequences available for alignments, identification of domains can be problematic. A method called expression of soluble proteins by random incremental truncation (ESPRIT) addresses this problem by high-throughput automated screening of tens of thousands of enzymatically truncated gene fragments. Rare soluble constructs are identified by experimental screening, and the boundaries revealed by DNA sequencing.
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Dates et versions

hal-01561714 , version 1 (13-07-2017)

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Philippe J Mas, Darren J Hart. ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.. Methods in Molecular Biology, 2017, 1586, pp.45-63. ⟨10.1007/978-1-4939-6887-9_4⟩. ⟨hal-01561714⟩
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