Tuning the allosteric regulation of artificial muscarinic and dopaminergic ligand-gated potassium channels by protein engineering of G protein-coupled receptors.

Abstract : Ligand-gated ion channels enable intercellular transmission of action potential through synapses by transducing biochemical messengers into electrical signal. We designed artificial ligand-gated ion channels by coupling G protein-coupled receptors to the Kir6.2 potassium channel. These artificial channels called ion channel-coupled receptors offer complementary properties to natural channels by extending the repertoire of ligands to those recognized by the fused receptors, by generating more sustained signals and by conferring potassium selectivity. The first artificial channels based on the muscarinic M2 and the dopaminergic D2L receptors were opened and closed by acetylcholine and dopamine, respectively. We find here that this opposite regulation of the gating is linked to the length of the receptor C-termini, and that C-terminus engineering can precisely control the extent and direction of ligand gating. These findings establish the design rules to produce customized ligand-gated channels for synthetic biology applications.
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Scientific Reports, Nature Publishing Group, 2017, 7, pp.41154. 〈10.1038/srep41154〉
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http://hal.univ-grenoble-alpes.fr/hal-01465674
Contributeur : Frank Thomas <>
Soumis le : lundi 13 février 2017 - 08:35:51
Dernière modification le : jeudi 12 avril 2018 - 11:50:02

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Christophe J Moreau, Jean Revilloud, Lydia Caro, Julien P. Dupuis, Amandine Trouchet, et al.. Tuning the allosteric regulation of artificial muscarinic and dopaminergic ligand-gated potassium channels by protein engineering of G protein-coupled receptors.. Scientific Reports, Nature Publishing Group, 2017, 7, pp.41154. 〈10.1038/srep41154〉. 〈hal-01465674〉

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