A three-dimensional movie of structural changes in bacteriorhodopsin.

Eriko Nango 1 Antoine Royant 2, 3 Minoru Kubo 1 Takanori Nakane 4 Cecilia Wickstrand 5 Tetsunari Kimura 1 Tomoyuki Tanaka 1 Kensuke Tono 1 So Song 1 Rie Tanaka 1 Toshi Arima 1 Ayumi Yamashita 1 Jun Kobayashi 1 Toshiaki Hosaka 6 Eiichi Mizohata 7 Przemyslaw Nogly 8 Michihiro Sugahara 1 Daewoong Nam 9, 10 Takashi Nomura 1 Tatsuro Shimamura 11 Dohyun Im 11 Takaaki Fujiwara 11 Yasuaki Yamanaka 11 Byeonghyun Jeon 10 Tomohiro Nishizawa 4 Kazumasa Oda 4 Masahiro Fukuda 4 Rebecka Andersson 5 Petra Båth 5 Robert Dods 5 Jan Davidsson 12 Shigeru Matsuoka 13 Satoshi Kawatake 13 Michio Murata 13 Osamu Nureki 4 Shigeki Owada 1 Takashi Kameshima 14 Takaki Hatsui 1 Yasumasa Joti 14 Gebhard Schertler 8 Makina Yabashi 1 Ana-Nicoleta Bondar 15 Jörg Standfuss 8 Richard Neutze 5 So Iwata 11
Abstract : Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.
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Science, American Association for the Advancement of Science, 2016, 354 (6319), pp.1552-1557. 〈10.1126/science.aah3497〉
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Soumis le : mardi 17 janvier 2017 - 08:37:54
Dernière modification le : lundi 8 octobre 2018 - 17:44:05

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Eriko Nango, Antoine Royant, Minoru Kubo, Takanori Nakane, Cecilia Wickstrand, et al.. A three-dimensional movie of structural changes in bacteriorhodopsin.. Science, American Association for the Advancement of Science, 2016, 354 (6319), pp.1552-1557. 〈10.1126/science.aah3497〉. 〈hal-01437084〉

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