A three-dimensional movie of structural changes in bacteriorhodopsin.

Eriko Nango; Antoine Royant; Minoru Kubo; Takanori Nakane; Cecilia Wickstrand; Tetsunari Kimura; Tomoyuki Tanaka; Kensuke Tono; So Song; Rie Tanaka; Toshi Arima; Ayumi Yamashita; Jun Kobayashi; Toshiaki Hosaka; Eiichi Mizohata; Przemyslaw Nogly; Michihiro Sugahara; Daewoong Nam; Takashi Nomura; Tatsuro Shimamura; Dohyun Im; Takaaki Fujiwara; Yasuaki Yamanaka; Byeonghyun Jeon; Tomohiro Nishizawa; Kazumasa Oda; Masahiro Fukuda; Rebecka Andersson; Petra Båth; Robert Dods; Jan Davidsson; Shigeru Matsuoka; Satoshi Kawatake; Michio Murata; Osamu Nureki; Shigeki Owada; Takashi Kameshima; Takaki Hatsui; Yasumasa Joti; Gebhard Schertler; Makina Yabashi; Ana-Nicoleta Bondar; Jörg Standfuss; Richard Neutze; So Iwata

doi:10.1126/science.aah3497

A three-dimensional movie of structural changes in bacteriorhodopsin.

Eriko Nango ¹ Antoine Royant ^{2, 3} Minoru Kubo ¹ Takanori Nakane ⁴ Cecilia Wickstrand ⁵ Tetsunari Kimura ¹ Tomoyuki Tanaka ¹ Kensuke Tono ¹ So Song ¹ Rie Tanaka ¹ Toshi Arima ¹ Ayumi Yamashita ¹ Jun Kobayashi ¹ Toshiaki Hosaka ⁶ Eiichi Mizohata ⁷ Przemyslaw Nogly ⁸ Michihiro Sugahara ¹ Daewoong Nam ^{9, 10} Takashi Nomura ¹ Tatsuro Shimamura ¹¹ Dohyun Im ¹¹ Takaaki Fujiwara ¹¹ Yasuaki Yamanaka ¹¹ Byeonghyun Jeon ¹⁰ Tomohiro Nishizawa ⁴ Kazumasa Oda ⁴ Masahiro Fukuda ⁴ Rebecka Andersson ⁵ Petra Båth ⁵ Robert Dods ⁵ Jan Davidsson ¹² Shigeru Matsuoka ¹³ Satoshi Kawatake ¹³ Michio Murata ¹³ Osamu Nureki ⁴ Shigeki Owada ¹ Takashi Kameshima ¹⁴ Takaki Hatsui ¹ Yasumasa Joti ¹⁴ Gebhard Schertler ⁸ Makina Yabashi ¹ Ana-Nicoleta Bondar ¹⁵ Jörg Standfuss ⁸ Richard Neutze ⁵ So Iwata ¹¹

1 RIKEN

2 IBS - UMR 5075 - Institut de biologie structurale

3 ESRF - European Synchrotron Radiation Facility

4 Department of Biological Sciences

5 Department of Chemistry and Molecular Biology - University of Gothenburg - Sweden

6 Division of Structural and Synthetic Biology

7 Department of Applied Chemistry

8 Division of Biology and Chemistry, Laboratory for Biomolecular Research

9 INRS-IAF - Institut Armand Frappier

10 Department of Physics

11 Department of Cell Biology, Graduate School of Medicine

12 Department of Chemistry - Ångström Laboratory

13 JST-Exploratory Research for Advanced Technology (ERATO)

14 Japan Synchrotron Radiation Research Institute, Kouto 1-1-1, Sayo, Hyogo 679-5148, Japan

15 Theoretical Molecular Biophysics, Department of Physics

Abstract : Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.

Keywords : specific radiation damage online Raman spectroscopy Branchiostoma lanceolatum fluorescent protein in crystallo optical spectroscopy

Type de document :

Article dans une revue

Science, American Association for the Advancement of Science, 2016, 354 (6319), pp.1552-1557. 〈10.1126/science.aah3497〉

Domaine :

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire / Biologie structurale [q-bio.BM]

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http://hal.univ-grenoble-alpes.fr/hal-01437084
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Soumis le : mardi 17 janvier 2017 - 08:37:54
Dernière modification le : jeudi 20 septembre 2018 - 13:16:05

A three-dimensional movie of structural changes in bacteriorhodopsin.

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