Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence-Dependence.

Abstract : Measles virus RNA genomes are packaged into helical nucleocapsids (NCs), comprising thousands of nucleo-proteins (N) that bind the entire genome. N-RNA provides the template for replication and transcription by the viral polymerase and is a promising target for viral inhibition. Elucidation of mechanisms regulating this process has been severely hampered by the inability to controllably assemble NCs. Here, we demonstrate self-organization of N into NC-like particles in vitro upon addition of RNA, providing a simple and versatile tool for investigating assembly. Real-time NMR and fluorescence spectroscopy reveals biphasic assembly kinetics. Remarkably, assembly depends strongly on the RNA-sequence, with the genomic 5' end and poly-Adenine sequences assembling efficiently, while sequences such as poly-Uracil are incompetent for NC formation. This observation has important consequences for understanding the assembly process.
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Angewandte Chemie (English Edition), John Wiley & Sons, 2016, 〈10.1002/anie.201602619〉
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http://hal.univ-grenoble-alpes.fr/hal-01341976
Contributeur : Frank Thomas <>
Soumis le : mardi 5 juillet 2016 - 11:18:56
Dernière modification le : lundi 19 février 2018 - 14:34:03

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Sigrid Milles, Malene Ringkjøbing Jensen, Guillaume Communie, Damien Maurin, Guy Schoehn, et al.. Self-Assembly of Measles Virus Nucleocapsid-like Particles: Kinetics and RNA Sequence-Dependence.. Angewandte Chemie (English Edition), John Wiley & Sons, 2016, 〈10.1002/anie.201602619〉. 〈hal-01341976〉

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