Protective effect of surfactant protein d in pulmonary vaccinia virus infection: implication of A27 viral protein.

Abstract : Vaccinia virus (VACV) was used as a surrogate of variola virus (VARV) (genus Orthopoxvirus), the causative agent of smallpox, to study Orthopoxvirus infection. VARV is principally transmitted between humans by aerosol droplets. Once inhaled, VARV first infects the respiratory tract where it could encounter surfactant components, such as soluble pattern recognition receptors. Surfactant protein D (SP-D), constitutively present in the lining fluids of the respiratory tract, plays important roles in innate host defense against virus infection. We investigated the role of SP-D in VACV infection and studied the A27 viral protein involvement in the interaction with SP-D. Interaction between SP-D and VACV caused viral inhibition in a lung cell model. Interaction of SP-D with VACV was mediated by the A27 viral protein. Binding required Ca2+ and interactions were blocked in the presence of excess of SP-D saccharide ligands. A27, which lacks glycosylation, directly interacted with SP-D. The interaction between SP-D and the viral particle was also observed using electron microscopy. Infection of mice lacking SP-D (SP-D-/-) resulted in increased mortality compared to SP-D+/+ mice. Altogether, our data show that SP-D participates in host defense against the vaccinia virus infection and that the interaction occurs with the viral surface protein A27.
Type de document :
Article dans une revue
Viruses, MDPI, 2013, 5 (3), pp.928-53
Liste complète des métadonnées

http://hal.univ-grenoble-alpes.fr/hal-01322444
Contributeur : Frank Thomas <>
Soumis le : vendredi 27 mai 2016 - 11:03:59
Dernière modification le : lundi 19 février 2018 - 14:34:03

Lien texte intégral

Identifiants

Collections

Citation

Julien Perino, Nicole Thielens, Erika Crouch, Danièle Spehner, Jean-Marc Crance, et al.. Protective effect of surfactant protein d in pulmonary vaccinia virus infection: implication of A27 viral protein.. Viruses, MDPI, 2013, 5 (3), pp.928-53. 〈hal-01322444〉

Partager

Métriques

Consultations de la notice

112